诱变葡萄体细胞胚获得同质四倍体植株的研究  

Study on Solid Tetraploid Plant Acquired from Inductive Mutation of Cell Plumule of Grapevine

作　　者：卢炳芝 李佩芬 于向荣 阎大成 郑旭芹 

机构地区：[1]山东省酿酒葡萄科学研究所,济南250100

出　　处：《果树科学》1997年第3期145-148,共4页Journal of Fruit Science

基　　金：山东省自然科学基金

摘　　要：运用植物细胞工程技术,诱导玫瑰香、胜利的花丝产生胚性愈伤组织,建立悬浮细胞系,获得不同发育阶段的胚状体;采用0.2%～0.6%的秋水仙素溶液处理不同发育阶段的胚状体96h,获得玫瑰香、胜利四倍体植株,经田间观察和细胞学鉴定,确认为同质四倍体新种质。Apply plant cell engineering techniques,induce the filaments of Muscat Hamburg and Pabieda to produce plumular callus and to set up a suspension cell system and thus obtain plumular substances in different stages.Then soak the plumular substances in different stages in 0.2%～0.6% colchicine solution for 96 hours and acquice the tetraploid plant of Muscat Hamburg and Pabieda which has been confirmed as a new kind of solid tetraploid through field observation and appraisement of cytology.

关 键 词：葡萄 胚状体 同质四倍体 诱变育种 

分 类 号：S663.103.5[农业科学—果树学]