东北地区蔬菜大棚内黄瓜枯萎病病株上镰孢菌的分离和鉴定  

Isolation and Identification of Fusarium Species from Cucumber Wilt Diseased Plants in Vegetable Greenhouses in Northeastern China

作　　者：杨晓贺[1] 吕国忠[2] 赵志慧[1] 刘丽莉[1] 姚贤民[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110161 [2]大连民族学院生物资源与环境研究所,辽宁大连116600

出　　处：《沈阳农业大学学报》2007年第3期308-311,共4页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金项目(30499340;30270014)

摘　　要：自东北三省蔬菜大棚内采集38株未嫁接黄瓜的枯萎病病株,采用组织分离法,共获得376株镰孢菌,根据形态鉴定,确定属于6种镰孢菌,其中尖孢镰孢(Fusarium oxysporum)占82.18%,木贼镰孢(F.equiseti)占9.31%,腐皮镰孢(F.solani)占4.52%,半裸镰孢(F.semitectum)占2.93%,串珠镰孢(F.moniliforme)和锐顶镰孢(F.acuminatum)分别只占0.53%。从辽宁省采集的8株长势弱的嫁接黄瓜植株根茎上分离到20株镰孢菌,分别为串珠镰孢(8株)、尖孢镰孢(7株)、半裸镰孢(4株)和木贼镰孢(1株)。376 isolates of Fusarium species were obtained from 38 cucumber plants showing wilt symptom in vegetable greenhouses in Northeastern China. Based on morphological characteristics, these isolates were identified to 6 species, namely F. oxysporum（82.1%）, F. equiseti（9.31%）, F. solani（4.52%）, F. semitectum（2.93%）, F. moniliforme（0.53%） and F. acuminatum（0.53%）. 20 Fusarium isolates were got from 8 grafted cucumber plants growing weakly in vegetable greenhouses in Liaoning Province, they belong to F. monilifotme（8 isolates）, F, oxyspornm（7 isolates）, F. semitectum（4 isolates） and F. equiseti（l isolate）.

关 键 词：黄瓜枯萎病 镰孢菌 形态鉴定 菌落 

分 类 号：S567.239[农业科学—中草药栽培]