六神丸可溶性砷形态的HPLC-ICP-MS研究  

Speciation analysis of solvable arsenic in Liushen pills by HPLC-ICP-MS

作　　者：吴倩[1] 梁琼麟[1] 罗国安[1] 王义明[1] 

机构地区：[1]清华大学化学系分析中心,北京100084

出　　处：《中国药科大学学报》2007年第4期332-335,共4页Journal of China Pharmaceutical University

基　　金：国家科技攻关计划资助项目(No.2004BA721A13);清华大学基础研究基金资助项目(No.JCqn2005038)~~

摘　　要：目的：建立中药复方中亚砷酸盐（As^Ⅲ）、砷酸盐（As^V）、一甲基砷（MMA）和二甲基砷（DMA）的HPLC-ICP-MS方法。方法：采用Hamilton PRP-X100阴离子交换柱，15mmol／L（NH4）2HP04为流动相（pH 6.0），建立了同时分析中药中As^Ⅲ，As^V、MMA和DMA的HPLC-ICP-MS方法，并对六神丸和雄黄中可溶性砷的形态进行了检测。结果：该分析方法在1-100ng／mL范围内线性关系良好，精密度和重复性良好，回收率为93％-106％。六神丸中几种药材与单味雄黄混合后，测得的可溶性砷含量均低于单味雄黄中的可溶性砷含量。蟾酥、牛黄、麝香、冰片、珍珠与雄黄混合后，砷溶出量比单味雄黄的砷溶出量依次减少93.6％，86.4％，71．3％，27．6％和14．7％。结论：六神丸复方中的几种单味药均有抑制雄黄中砷溶出的作用。推测是降低雄黄毒性作用的可能途径之一。Aim：To establish a method for the determination of arsenite （As^Ⅲ ）, arsenate （As^V）, dimethylarsinic acid （DMA） and monomethylarsonate （MMA） in the traditional Chinese medicine （TCM） by HPLC-ICP-MS. Methods： The method for simultaneous determination of As^Ⅲ , As^V , MMA and DMA in TCM was established by using a Hamilton PRP-X100 anion exchange column with the mobile phase of 15 mmol/L （NH4）2HPO4 （pH 6.0）, and the solvable arsenic in Liushen pills and realgar were detected. Results： In four arsenic species, showed a good linearity in the concentration range of 1 - 100 ng/mL with accepted accuracy and precision. Calculated recoveries were 93% - 106%. Contents of solvable arsenic in realgar mixed with other components in Liushen pills were lower than those in realgar only. Components of toad venom （Bufo bufo gargarizans Cantor）, bezoar （Bos taurus domesticus Gmelin）, musk （ Moschus berezorskii Fleror）, borneol （ Dryobalanops aromatica Gaertner. f. ） and pearl （ Pteria martensii Dunker） decreased the contents of solvable arsenic in realgar by 93.6%, 86.4%, 71.3%, 27.6% and 14.7%, respectively. Conclusion： Components in Liushen pills could reduce the solubility of arsenic in realgar, which might be one of the mechanisms responsible for the decreased toxicity of realgar.

关 键 词：六神丸 雄黄 砷形态分析 HPLC-ICP-MS 

分 类 号：R286.0[医药卫生—中药学]