五味地龙汤对哮喘豚鼠血清白细胞介素-4的影响  

Effect of Schisandra Fruit and Earthworm Decoction on Serum Interleukine-4 in Experimental Asthma Guinea Pigs

作　　者：王文英[1] 张德新[1] 涂献玉[1] 张渊[1] 杜亚明[1] 李祥华[1] 

机构地区：[1]长江大学医学院,湖北荆州434000

出　　处：《长江大学学报（自科版）（下旬）》2007年第2期109-111,共3页Journal of Yangtze University

基　　金：湖北省教育厅科学研究项目(项目编号:B200512006)

摘　　要：目的:观察五味地龙汤对哮喘血清白细胞介素-4(IL-4)水平的影响,以探讨其治疗机制。方法:取豚鼠48只,随机分为正常对照组,哮喘模型组,地塞米松组,小青龙汤组,五味地龙汤大、小剂量组,每组8只。卵白蛋白(OVA)腹腔注射致敏,气道吸入激发建立哮喘动物模型。按豚鼠IL-4 ELISA试剂盒说明,采用双抗夹心ELISA法,测定血清IL-4含量。结果:哮喘模型组豚鼠OVA致敏并激发后出现活动减少,反应迟钝,毛色失去光泽,呼吸次数增加,呼吸困难。IL-4测定结果显示,五味地龙汤大剂量组豚鼠血清IL-4浓度为(9.27±0.86)pg·ml-1,五味地龙汤小剂量组血清IL-4浓度为(10.77±1.17)pg·ml-1,地塞米松组血清IL-4含量(9.40±2.91)pg·ml-1,与哮喘模型组(13.37±1.86)pg·ml-1比较,差异均有显著性(P<0.01)。小青龙汤组血清IL-4含量为(10.94±1.30)pg·ml-1,与哮喘模型组比较,差异亦有显著性(P<0.05)。各药物组与正常对照组(10.45±0.62)pg·ml-1比较,均无统计学意义(P>0.05)。结论:五味地龙汤能降低哮喘动物血清IL-4含量。这可能是五味地龙汤能抑制炎症介质的释放、治疗气道炎症的机制之一。Objective: To explore the effective mechanism of Schisandra fruit and Earthworm decoction on asthma.Methods:Forty-eight guinea pigs were randomly divided into six groups: the control group,the model group,the dexamethasone group,the Xiaoqinglongtang group,the schisandra fruit and earthworm decoction large and small dosage groups,8guinea pigs in each group. The guinea pigs were sensitized with 10% ovalbumin (OVA) by a combination of intraperitional injection and repeated with 1% OVA intranasal challenge to establish asthma model. The level of serum interleukine-4 (IL-4) was determined by double antibody sandwich ELISA method with the guinea pigs IL-4 kit. Results: The model guinea pigs group repeated with OVA intranasal challenge moved about decreasing,reaction retardation,lustre of coat of hair lost,tachypnea and dyspneic respiration,but other groups were a good state. The assay of serum IL-4 in large dosage group of Schisandra fluit and Earthworm decoction (9.27±0.86) pg·ml-1,small dosage group of Schisandra fluit and Earthworm decoction (10.77±1.17) pg·ml-1 and dexamethasone group (9.40±2.91) pg.ml-1 was significantly decreased as compared to the model group (13.37±1.86) pg·ml-1 (all P<0.01). And xiaoqinglong tang group (10.94±1.30)pg·ml-1 was significantly decreased as compared to the model group ,too(P<0.05).There was no difference among the Schisandra fruit and Earthworm decoction groups,dexamethasone group,xiaoqinglong tang group and control group (10.45±0.62) (all P>0.05).Conclusion: It was demonstrated that Schisandra fruit and Earthworm decoction can reduce the level of serum IL-4 in asthmatic guinea pigs. This might be an important cause that the decoction can inhibit inflammatory media to release and treat airway inflammation.

关 键 词：五味地龙汤 哮喘 白细胞介素4 细胞因子 

分 类 号：R562.65[医药卫生—呼吸系统]