血管内皮细胞组织特异性表达人CD59转基因小鼠抗超急性排斥反应能力  

Studies on the endothelial expression of functional human CD59 in transgenic mice and its protection against hyperacute rejection

作　　者：张志宏[1] 马腾骧[1] 王广有[1] 张玥[1] 李胜芝[1] 李光三[2] 刘思金[2] 

机构地区：[1]天津市泌尿外科研究所,天津300211 [2]中国科学院遗传与发育生物研究所

出　　处：《天津医科大学学报》2007年第2期146-148,157,共4页Journal of Tianjin Medical University

基　　金：天津市自然科学基金项目资助(013611011)

摘　　要：目的:检测血管内皮细胞组织特异性表达人CD59转基因小鼠抗超急性排斥反应能力。方法:采用Langendorff心脏灌注装置,用20%稀释人血清灌注转基因小鼠离体心脏,观察其抗超急排斥反应能力。免疫组化方法检测灌注后心脏组织人IgM沉积情况。结果:与普通鼠对比,转基因小鼠离体心脏在用20%稀释人血清灌注期间心脏存活时间明显延长,且60min灌注期间内做功仍维持在最大值20%以上,显示转基因小鼠具有一定抗超急排斥反应能力。免疫组化显示C3c(免疫组化一抗)在转基因组心肌组织间、血管管壁仅有少量沉积,普通小鼠组大量沉积,显示普通小鼠心脏遭受较为强烈的超急排斥反应损伤。结论:血管内皮细胞组织特异性表达人CD59转基因小鼠有一定的抗超急排斥反应能力。Objective： To investigate the ability from the endothelial expression of functional human CD59 in transgenic mice to protect against human hyperacute rejection. Methods： An Langendorff ex vivo perfusion model was used to compare hearts from the hCD59 mice perfused with 20% human serum with wild-type hearts and the ability against hyperacute rejection was observed. Immunohistochemical survey was performed to detect the deposition of hIgM on the fresh-frozen tissue sections of perfused mouse hearts. Results： These endothelial-specific hCD59 hearts displayed prolonged survival compared to wild-type hearts during perfusion with 20% human plasma and maintained approximately 20% maximum work in 60 rains, showing some ability against hyperacute rejection. Wild-type hearts showed evidence of tissue damage, with disruption of muscle bundles and a more scattered staining pattern, whereas hearts from transgenic group were relatively intact morphol- ogically and weaker focal staining. Conclusion： The mice with hCD59 tissue-specifically expressed on vascular endothehum show some abihty against hyperacute rejection.

关 键 词：异种移植 排斥反应 转基因 CD59 内皮细胞 离体心脏灌注 小鼠 

分 类 号：Q7[生物学—分子生物学] R392.4[医药卫生—免疫学]