灵敏的单一溴酚蓝测定尿蛋白  

A Simple Sensitive Bromophenol Blue Reagent to Determine Uric Protein

作　　者：方芳[1] 戚玉海[1] 张锋[1] 贺媛媛[1] 赵长容[1] 曹建明[1] 

机构地区：[1]温州医学院环境与公共卫生学院,浙江温州325035

出　　处：《广东微量元素科学》2007年第6期37-39,共3页Trace Elements Science

基　　金：温州医学院校内资助课题(200601029)

摘　　要：在pH3．0的柠檬酸-柠檬酸钠缓冲液中，用溴酚蓝作显色剂，建立了一种快速、简便、灵敏、稳定的测定尿蛋白的分光光度法。该法显色络合物的最大吸收波长为620nm，线性范围0-4．0g／L，批内变异系数为0．52％，批间变异系数为1．27％，日间变异系数为1．51％，回收率为98．0％～99．3％，与邻苯三酚红比较有良好相关性，回归方程为Y=0．030＋0．830z，相关系数为r=0．9798。A simple, sensitive and stable method to determination of protein in urine by spectrophotometry with bromophenol blue （BPB） was established. In the pH 3.0 citric acid buffer system, the maximum absorption of the complex compound was at 620 nm. The linearity was 0 ～ 4. 0 g/L. The within run, between run and between day CV was respectively 0. 52% , 1.27% and 1.51%. The recoveries ranged from 98.0% to 99.7%. The correlation between results by this method （y） and pyrogallol red （x） was y = 0. 030 ＋ 0. 830 x, r = 0. 979 8 .

关 键 词：溴酚蓝 尿蛋白 分光光度法 

分 类 号：O657.32[理学—分析化学]