脊神经节与心肌细胞联合培养中降钙素基因相关肽的合成  

SYNTHESIS OF CALCITONIN GENE-RELATED PEPTIDE IN CO-CULTURES OF SPINAL GANGLIA AND CARDIAC MYOCYTES

作　　者：李振中[1] 于彦铮[1] 彭裕文[1] 刘才栋[1] 

机构地区：[1]上海医科大学解剖学教研室,上海200032

出　　处：《解剖学杂志》1997年第2期133-135,共3页Chinese Journal of Anatomy

摘　　要：用免疫组织化学法研究了新生大鼠脊神经节与心肌细胞联合培养中降钙素基因相关肽的合成.在脊神经节与心肌细胞联合培养72小时的标本中,降钙素基因相关肽免疫反应呈阴性.在联合培养96小时的标本中,出现了许多降钙素基因相关肽免疫反应阳性神经纤维:表明在体外联合培养96小时以后,神经递质的合成才发育完成.The synthesis of calcitonin gene-related peptide (CGRP) was examined by immunohis-tochemical method in the co-cultures of spinal ganglia and cardiac myocytes. CGRP-immunoreac-tive nerve fibers were not observed in the neuromuscular co-cultures at 72 hours of culture age, but there were many CGRP-immunoreactive nerve fibers in the neuromuscular co-cultures at 96 hours of culture age. The results indicate that the synthesis of neurotransmitter is not maturated in the neuromuscular co-cultures until 96 hours of culture age.

关 键 词：脊神经节 心肌细胞 降钙素 基因相关肽 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]