ELISA竞争抑制法检测Anti-HBe试剂盒共系统检测HBeAg结果分析  

Analysis on the Detection of Sample HBeAg by ELISA in A Common Measurement System with Anti-HBe Using Its EIA Reagent Kits of A Competitive Inhibition Method

作　　者：李宏杰[1] 朱峰[1] 程家坤[2] 何从贤 

机构地区：[1]安徽省淮南新华医疗集团新华医院临床检验中心,安徽淮南232052 [2]安徽省淮南新华医疗集团新华医院核医学科,安徽淮南232052

出　　处：《中国现代医生》2007年第07X期15-17,共3页China Modern Doctor

摘　　要：目的探讨用竞争抑制法检测Anti-HBeEIA[预包被纯化乙肝e抗原]商品试剂盒共系统检测HBeAg的可行性及结果判定方法。方法用竞争抑制法检测Anti-HBeELISA试剂盒共系统检测1000份随机血清(浆)标本Anti-HBe与HBeAg,并与常规ELISA双抗体夹心法检测HBeAg试剂盒检测HBeAg作平行对照。结果ELISA共系统检测HBeAg,其阳性检测孔显色较Anti-HBe阴性对照孔(或HBeAg阴性参比对照孔)明显加深,易于目测;其比色COV易于设定(或使用HBeAg临界值血清),与常规ELISA检测HBeAg比色判定无显著性差异[配对计数资料比较的χ2检验:相关检验均为P<0.005,υ=1,a=0.05;优劣检验依次为HBeAg临界值血清(1)P>0.9000,υ=1,a=0.05、中和(竞争)抑制法检测Anti-HBeEIA商品试剂盒中Anti-HBe阴性对照(2)0.100>P>0.050,υ=1,a=0.05、ELISA共系统检测HBeAgCOV(3)0.100>P>0.050,υ=1,a=0.05,但χ21<χ22<χ23]。结论用竞争抑制法检测Anti-HBeELISA商品试剂盒能够兼测HBeAg,在有优质现代酶标仪的实验室,可以省去常规ELISA双Ab夹心法检测HBeAg试剂盒,实用价廉,值得推广。Objective To investigate the practicality and how to deal with the results of detecting sample HBeAg by ELISA in a common measurement system with Anti-HBe using its EIA（coating purified HBeAg） reagent kits of a competitive inhibition method. Methods HBeAg of 1000 random serum （or plasma） samples was measured by ELISA in a common measurement system with Anti-HBe using its ELISA reagent kits of a competitive inhibition method and the results were aslo detected by a routine ELISA of HBeAg using its EIA reagent kits of a double？antibody sandwich method as a control. Results The results of detecting sample HBeAg in the common measurement system are easy to be differentiated with one＇ s eyes, its positive hole colours obviously heavier than negative control of Anti-HBe （or negative reference control of HBeAg） ; its cut off value（COV） is similar to a routine ELISA of HBeAg and easier to be set up （or HBeAg serum of COV was used） and the HBeAg quanlitative assay o f OD measurements was no significant defference between ELISA in a common measurement system and a routine ELISA of HbeAg [ χ^2test of enumeration data with paried design：correlation test all are P 〈0.005, v =1,a=0.05;difference test is successively HBeAg serum of COV （1） P 〉0.900, v = 1,a=0.05 ;Anti-HBe negative control of its EIA reagent kits of a nuetralizing and competitive inhibition method（2） 0.100〉P 〉0.050, v = 1,a=0.05 ; COV of HBeAg EIA in a common measurement system（3） 0.100〉P 〉0.050, v = 1,a=0.05, however χ^21 〈 χ^22 = 〈 χ^23）. Conclusion The detection of sample HBeAg by ELISA in a common measurement system with Anti-HBe using its ELISA reagent kits of a competitive inhibition method is practicable,if a modern and high quality analizer of ELISA were installed in one＇ s own clinical laboratory, a routine ELISA a double-antibody sandwich method reagent kits of HBeAg should be ommitted.It is of practicable and cheap and is worthy of popularizing.

关 键 词：酶联免疫吸附试验 竞争抑制法 双抗体夹心法 共系统检测 乙型肝炎病毒E抗原 

分 类 号：R446.61[医药卫生—诊断学]