有机磷杀虫剂乐果对乙酰胆碱受体通道的阻断作用  

Blocking Effects of Dimethoate on Acetylcholine Receptor Channels

作　　者：徐海滨[1,2] 贺锡雯[1,2] 谢佐平 何凤生[1,2] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所 [2]清华大学生物科学与技术系

出　　处：《卫生研究》1997年第3期154-158,共5页Journal of Hygiene Research

基　　金：卫生部重点研究课题;国家自然科学基金

摘　　要：在非洲有爪蟾蜍胚胎神经和肌肉细胞上，用膜片钳技术研究了有机磷杀虫剂乐果对乙酰胆碱受体（ＡＣｈＲ）通道的作用，发现它对自发微终板电流（ＭＥＰＣ）有双重作用，低剂量（２１．６μｍｏｌ／Ｌ）增加其幅度和频率，高剂量（２３７μｍｏｌ／Ｌ）则相反。１４６μｍｏｌ／Ｌ乐果缩短通道开放时间降低开放概率。对ＡＣｈＲ通道电流幅度和细胞膜钠、钾通道电流无影响。提示乐果为ＡＣｈＲ通道开放阻断剂。本实验从分子水平上为有机磷杀虫剂对ＡＣｈＲ的直接作用提供了依据。The patch clamp technique was used to study the inhibitory effect of dimethoate,an organophosphrous pesticide,on the membrane of embryoinc Xenopus musle cells in culture.Using whole cell clamp and cell attached clamp,we found that the direct inhibitory effects of dimethoate was evidenced on both miniature end plate currents(MEPC) and single channel currents.Dimethoate increased the amplitude and frequency of MEPC at a lower concentration (21 6μmol/L) while both decreased at a higher concentration(237μmol/L).The dimethoate at a concentration of 146μmol/L shortened the opening time and decreased the opening probability of the single channel.The results show that dimethoate is an AChR channel blocker.

关 键 词：有机磷杀虫剂 乙酰胆碱 受体 膜片钳 农药中毒 

分 类 号：R595.402[医药卫生—内科学]