五苓散对阿霉素肾病大鼠肾组织内皮素A型受体表达的影响  

Effect of Wuling Powder on expression of endothelin receptor A of renal tissue in rats with adriamycin-induced nephrosis

作　　者：何岚 蔡宇[2] 陈朝晖[1] 胡海燕[3] 王宁生[1] 

机构地区：[1]广州中医药大学临床药理研究所,广东广州510405 [2]暨南大学药学院,广东广州510632 [3]中山大学药学院,广东广州510080

出　　处：《中成药》2007年第7期963-966,共4页Chinese Traditional Patent Medicine

摘　　要：目的:探讨五苓散(猪苓、泽泻、白术、茯苓、桂枝)减轻阿霉素(ADR)肾病大鼠蛋白尿可能存在的分子机制。方法:制备ADR肾病大鼠模型,常规灌胃给予五苓散,运用免疫组织化学的方法检测五苓散对模型动物肾组织内皮素A型受体(endothelin receptor A,ETAR)蛋白表达的影响;运用逆转录-聚合酶链反应(reverse transcription-polymerase chain reac-tion,RT-PCR)检测对ETARmRNA表达的影响。结果:模型动物肾组织内皮素A型受体(ETAR)蛋白及ETARmRNA的表达显著增高(模型组与空白对照组比较,P<0.05),五苓散组动物肾组织ETAR蛋白及ETARmRNA的表达较模型组显著降低(给药组与模型组比较,P<0.05)。结论:五苓散可抑制ADR肾病大鼠肾组织ETAR蛋白及mRNA的高表达,这可能是其减轻ADR肾病大鼠蛋白尿的作用机理之一。AIM ： To investigate the possible molecular mechanism of Wuling Powder （ Polyporus, Rhizoma alismatis, Rhizoma atractylodis macrocephalae, Poria, Ramulus cinnamoni） for reducing proteinuria in rats with Adriamycin-induced nephrosis ADR rat. METHODS ： After orally administrated for four weeks, ADR rats were measured the expression of endothelin receptor A （ETAR） in renal tissue, by immunohistochemistry and RT-PCR method. RESULTS ： The expressions of ETAR protein and mRNA in ADR rats increased obviously （ in comparison with control rats, P 〈 0.05 ）, which decreased in Wuling Powder administrated rats （ in comparison with ADR rats, P 〈 0.05 ）. CONCLUSION ： Wuling Powder could inhibit the enhanced renal expression of ETAR protein and mRNA in ADR rats, contributed to reduction in proteinuria.

关 键 词：五苓散 阿霉素 内皮素受体 

分 类 号：R285.6[医药卫生—中药学]