HPLC测定复方丙戊酰胺胶囊中苯妥英钠和五味子醇甲的含量  

Determination of phenytoin sodium and schisandrin in Compound Valpromide Capsule by HPLC

作　　者：付萍萍[1] 景浩然[1] 赵志伟[1] 

机构地区：[1]河北保定市药品检验所,河北保定071051

出　　处：《中成药》2007年第7期1008-1010,共3页Chinese Traditional Patent Medicine

摘　　要：目的:建立复方丙戊酰胺胶囊(丙戊酰胺,苯妥英钠,五味子,维生素B6等)中苯妥英钠和五味子醇甲含量的HPLC测定方法。方法:采用HypersilBDSODS色谱柱,流动相为甲醇-0.02mol/L磷酸二氢钾溶液(60∶40),检测波长为254nm。结果:苯妥英钠和五味子醇甲分别在4.50μg～72.0μg(r=1.0000)和0.0244～0.391μg(r=0.9999)范围内呈线性关系。平均回收率(n=9)分别为100.21,102.32,RSD分别为1.3,0.5。结论:本法简便、可靠、准确,可用于该制剂的质量控制。AIM： To establish a HPLC method for determining phenytoin sodium and sehisandrin in Compound Valpromide Capsule （ Valpromide, phenytoin sodium, Fructus Schisandrae Chinensis, VB6, etc ）. METHODS ： A Hypersil BDS ODS column was used. The mobile phase was MeOH -0.02 mol/L potassium Dihydrogen Phosphate （60： 40）. The detection wavelength was at 254 nm. RESULTS： The linear range of phenytoin sodium was within 4.50 μg-72.0 μg（r = 1. 000 0） ,The linear range of schisandrin was within 0.024 4 μg-0.391 μg（r =0. 999 9）. The average recoveries （n = 9） were 100.21% and 102.32%, respectively. RSD were 1.3 % and 0.5 %, respectively. CONCLUSION ： The method is simple, reliable, accurate and can be applied to the quality control of the preparation.

关 键 词：苯妥英钠 五味子醇甲 复方丙戊酰胺胶囊 HPLC 

分 类 号：R927.2[医药卫生—药学]