流式细胞术检测冠心病患者血小板表面糖蛋白的变化  

Detect the glycoprotein of platelet with CHD by FCM

作　　者：郭壮波[1] 韦建瑞[1] 张文超[1] 蒋作锋[1] 张锐[1] 黄小云[1] 

机构地区：[1]暨南大学医学院附属第四医院.广州市红十字会医院,510220

出　　处：《广州医药》2007年第1期17-19,共3页Guangzhou Medical Journal

基　　金：2005年广东省科技厅基金(53064);2004年广州市医药卫生科技重点项目(2004Z006)资助

摘　　要：目的观察冠心病患者及其亚组的血小板膜P选择素(CD62P)和糖蛋白Ⅱb/Ⅲa(PAC-1)的变化及其意义。方法采用流式细胞仪三色荧光标记技术来测定急性冠脉综合征、慢性心肌缺血综合征患者及22名健康对照组的血小板表面糖蛋白(CD62p/PAC-1)的表达率,并比较其变化。结果病例组的血小板表面糖蛋白表达率显著高于对照组,其中急性冠脉综合征患者的PAC-1:(15.54±6.43)%,CD62p:(9.83±6.06)%,与对照组相比(P<0.001),慢性心肌缺血综合征PAC-1:(5.38±2.62)%,CD62p:(11.00±6.37%),较对照组也有显著升高(P<0.001)。急性冠脉综合征组的PAC-1水平显著高于慢性心肌缺血综合征CIS组P<0.001。结论冠心病患者处于血小板高度活化状态,其中急性冠脉综合征患者血小板处于活化的早期阶段,而慢性心肌缺血综合征患者血小板处于慢性激活状态,CD62P和PAC-1是血小板功能检测非常敏感的指标。Objective To investigate the changes in P-selection （CD62P） and glycoprotein Ⅱ b/Ⅲ a （ PAC-1 ） on platelets in patients with coronary heart disease. Methods CD62p, PAC-1 from whole blood samples were measured by FCM in 22 normal people and 75 patients with CHD, which is grouped into ACS and CIS by symptom EKG CK-MB and cTNT. Results The platelet CD62p and PAC-1 expression in CHD is 10. 36 ± 6. 15% and 10. 27 ±7.12% evidently higher than control （P 〈0. 001 ） , In addition, the platelet PAC-1 expression is higher in ACS than CIS and control before and after treatment. Conclusion The platelet in CHD is under activated ACS group is in the early stage of activation CD62p and PAC-1 is the sensitive marker of platelet activation.

关 键 词：冠心病 血小板表面糖蛋白 流式细胞术 

分 类 号：R541.4[医药卫生—心血管疾病]