微生物3-甾酮-△^1-脱氢酶的研究进展  

The Research Advances on 3 -Ketosteroid-△^1- Dehydrogenase of Microorganism

作　　者：李玉[1] 田琳[1] 刘逸寒[1] 路福平[1] 

机构地区：[1]天津市工业微生物重点实验室天津科技大学生物工程学院,天津300222

出　　处：《药物生物技术》2007年第2期149-152,共4页Pharmaceutical Biotechnology

摘　　要：3-甾酮-△1-脱氢酶(KSDH)属于黄素蛋白酶类,位于细胞膜上,能在3-甾酮化合物的C1和C2之间引入双键,提高原有底物的抗炎活性,在甾体药物的生产上发挥着重要作用。文章对已报道的不同微生物来源的KSDH进行比较分析,着重介绍了关于KSDH基因表达和敲除方面的研究,并对该酶在甾体转化方面的应用做一简要总结。3 - ketosteroid -△^1- dehydrogenase（KSDH） belongs to flavoprotein, bound to the membrane of the cell. It can introduce double bond to the C1 and C2 of 3 - keto - steroids, which enhanced the antiinflammatory activity of substrates. There fore it played an important role in the steroid pharmaceutical industry. The comparison on amino acid sequences of 3 - ketosteroid-△^1- debydrogenase from different microorganisms and the latest research on gene expression and targeted disruption were focused in this review. And the application of KSDH in steroid transformation was also summarized in this article.

关 键 词：3-甾酮-△^-1-脱氢酶 表达 敲除 甾体转化 

分 类 号：Q554.9[生物学—生物化学]