红栀子愈伤组织诱导研究  

Study on Callus Induction of Gardenia jasminonides Ellis

作　　者：孟志卿[1] 

机构地区：[1]孝感学院湖北省作物病害监测和安全控制实验室,湖北孝感432000

出　　处：《安徽农业科学》2007年第20期6044-6044,6332,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为了研究红栀子组织培养的最佳条件,为栀子黄色素的工业化生产提供理论依据。[方法]以红栀子茎尖、茎段和幼嫩叶片为外植体,在MS、1/2MS和1/4MS3种培养基上进行培养试验,研究不同外植体、培养基、激素浓度和消毒时间对诱导愈伤组织的影响。[结果]红栀子茎尖和幼嫩叶片的诱导效果明显优于茎段。在3种培养基中,MS培养基更有利于愈伤组织的产生和生长。在MS培养基中添加2,4-D时,2.0mg/L为诱导红栀子茎尖愈伤组织的最适浓度。用升汞消毒,茎尖、茎段和幼嫩叶片的最佳消毒时间分别为9、12和7min。[结论]MS+2,4-D2.0mg/L+6-BA1.0mg/L为红栀子幼嫩叶片愈伤组织的最佳培养基,且10d后形成愈伤组织。[Objective] The research aimed to investigate the optimum conditions for the tissue culture of Gardeniajasminonides Ellis so as to provide the theoretical basis for the industrialization production of gardenia yellow pigment. [Method] In the callus culture experiment with the stem tips, stem segments and tender leaves of G.jasminonides as explants, 3 kinds of culture medium of MS, 1/2MS and 1/4MS were used to study the effects of different explants, culture medium, hormone concentrations and disinfection time on the callus induction. [Result] The induction effects of stem tips and tender leaves were obviously better than that of stem segments. Among 3 kinds of culture medium, MS culture medium was more favorable for the induction and growth of callus. When 2,4 -D was supplemented to MS culture medium, the suitable concentration for inducing the callus of stem tips was 2.0 mg/L. When mercuric chloride was used to sterilize, the optimum sterilization time for stem tips, stem segments and tender leaves were 9, 12 and 7 min respectively. [Conclusion] The optimum culture medium for the callus of tender leaves of G. jasminonides was MS＋2,4-D 2.0 mg/L＋6-BA 1.0 mg/L and the callus was formed after 10 days.

关 键 词：红栀子 愈伤组织 诱导 

分 类 号：Q943.1[生物学—植物学]