荷兰百合索尔邦的离体组织培养  

Tissue Culture in vitro of Holland Lily Sorbonne

作　　者：陈学林[1] 李卫锋[1] 黄海涛[2] 

机构地区：[1]西北师范大学生命科学院,甘肃兰州730070 [2]西南科技大学生命科学与工程学院,四川绵阳621002

出　　处：《安徽农业科学》2007年第20期6045-6046,共2页Journal of Anhui Agricultural Sciences

基　　金：西南科技大学与中国工程物理研究院横向合作研究课题(2003205)

摘　　要：[目的]寻找荷兰百合索尔邦最佳离体培养途径,大幅度提高其繁殖速度。[方法]利用荷兰百合索尔邦的不同组织作外植体进行离体培养,进行不同分化培养基对不同外植体分化效果的影响,不同激素浓度对索尔邦百合鳞片诱导出芽效果的影响,不同部位的百合鳞片对诱导出芽效果的影响,不同激素浓度对百合丛生芽增殖的影响和生根与移栽等研究。[结果]结果表明:组培荷兰百合索尔邦以鳞茎为最佳外植体,以MS+6-BA 2.0mg/L+IAA 1.0mg/L的培养基诱导丛生芽效果最好,其诱导率为96.7%;鳞片诱导中鳞茎中部表现出较大的器官发生潜力;百合继代增殖培养以MS+6-BA 1.0mg/L+IAA 0.4mg/L培养基为宜,组培苗在MS+IAA 0.5mg/L的培养基中较易生根,生根时间约25d。[结论]该研究为百合的辐射育种提供了技术支持。[Objective] The optimum culture route in vitro for Holland lily variety Sorbonne were searched, which could improve propagation rate. [Method] The bulb, tip bud, stem section and leaf of Holland lily variety Sorbonne were selected for in vitro culture. [Result] Bulb was the best explants and the optimum multiple bud induction medium was MS ＋6-BA 2.0 mg/L ＋ IAA 1.0 mg/L with induction rate of 96.7 %. The central section of bulb displayed big organogenesis potential. The optimum regeneration and rooting medium for lily was MS ＋BA 1.0 mg/L＋ IAA 0.4 mg/L and MS ＋IAA 0.5 mg/L, respectively, with rooting time of about 25 d. [Conclusion] Technical support were provided for the studying of lily breeding.

关 键 词：百合 索尔邦 组织培养 

分 类 号：Q943.1[生物学—植物学]