木霉菌REMI转化子解磷筛选及其解磷效果分析  

Phosphate-dissolving Screening and Effective Analysis of Trichoderma REMI Transformant

作　　者：菅丽萍[1] 刘力行[2] 陈云鹏[2] 薛春生[1] 陈捷[2] 

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110161 [2]上海交通大学农业与生物学院,上海201101

出　　处：《安徽农业科学》2007年第20期6179-6180,6191,共3页Journal of Anhui Agricultural Sciences

基　　金：农业部948项目(2006-G54(A));上海市科委中法合作项目(063407060);上海市科委崇明重大专项(05DZ19104)

摘　　要：[目的]为了获得具有防病和解磷双重功能的木霉菌REMI转化子。[方法]从康氏木霉菌(Trichoderma koningii)T30及其60株REMI(限制性内切酶介导的基因整合技术)转化子中筛选出3株解磷作用较好的转化子,并研究不同碳、氮源对转化子TK!46解磷作用的影响。[结果]结果表明,碳源对转化子解磷能力影响较小,而氮源的作用较大,其中转化子在解磷过程中最适氮源是氨态氮。[结论]该研究为研制多功能木霉剂奠定了基础,但完全揭示木霉菌解磷机理仍需进一步研究。[Objective] The aim of this study was to obtain Trichoderma REMI transformant with double functions of disease prevention and phosphate-dissolving. [Method] Three preferable phosphate -dissolving transformants were screened out from Trichoderma konigii T30 and its 60 REMI（rcstriction enzyme mediated DNA integration ）transformants, Furthermore, the effect of different carbon and nitrogen sources on phosphate solubilization of TK-46 was investigated. [Result] Result showed that there was minor influence of carbon sources on the phosphate solubilization. But significant influence of nitrogen sources was found on the phosphate solubilization of Trichoderma. Comparatively NH4^＋-N was subjected to be utilized more easily by Trichoderma transformant than NO3-N during the solubilization of phosphate. [Conclusion] This research provided basis for the study of Trichoderma with multiple functions. But there still needed further study to reveal the phosphate-dissolving mechanism of Trichoderma. Key words

关 键 词：木霉菌REMI转化子 解磷 碳源 氯源 

分 类 号：Q949[生物学—植物学]