枯否细胞摄取大肠杆菌脂多糖的区域性差异  

Difference between periportal and pericentral Kupffer cells in uptaking lipopolysaccharides in rats

作　　者：陈贤明[1,2] 刘近春[1,2] 许瑞龄 马学惠 赵元昌 韩德五 

机构地区：[1]山西医科大学肝病研究所 [2]山西医科大学第一医院

出　　处：《新消化病学杂志》1997年第5期280-282,共3页

基　　金：山西省青年科技基金;山西省自然科学基金

摘　　要：目的探讨肝脏中心静脉区域及门静脉区域枯否细胞对门静脉来源细菌脂多糖摄取作用的差异．方法Ｗｉｓｔａｒ大鼠随机分成正常对照组（ｎ＝６）及ＧｄＣｌ３注射组（ｎ＝８）．实验开始前１６ｈＧｄＣｌ３注射组大鼠尾静脉注射ＧｄＣｌ３以选择性抑制门静脉区域枯否细胞吞噬功能．二组大鼠均经由门静脉内注入大肠杆菌脂多糖２０μｇ／１００ｇ体重，０，２，５，１０，３０，６０ｍｉｎ后取肝脏固定，应用单克隆抗脂多糖抗体，采用免疫荧光及共焦点激光扫描技术，对脂多糖在肝脏枯否细胞内的时相性分布进行了观察．结果正常大鼠门静脉区域枯否细胞于脂多糖注射２ｍｉｎ后其胞浆内抗脂多糖荧光活性增高达峰值（１２３６０±１１４１）μＦＩ／μｍ２，中心静脉区域枯否细胞于注射后５ｍｉｎ达峰值（９６２０±４７１）μＦＩ／μｍ２，前者荧光增高幅度显著高于后者水平（Ｐ＜００１）．ＧｄＣｌ３注射大鼠中心静脉区域枯否细胞于注射脂多糖后２ｍｉｎ抗脂多糖荧光强度达峰值（１００６０±７６２）μＦＩ／μｍ２，但峰值水平与正常对照组相比无明显差异（Ｐ＞００５）．ＧｄＣｌ３注射组大鼠门静脉区域枯否细胞抗脂多糖荧光强度各时间点均无明显变化．结论门静脉区域及中心静脉区域枯否?AIM To reveal the difference of Kupffer cells in the periportal and pericentral regions in the liver to uptake lipopolysaccharides (LPS) injected by the portal vein. METHODS Male Wistar rats were divided into two groups: normal control group ( n =6) and GdCl 3 treated group ( n =8). Sixteen hours before the experiment, rats of GdCl 3 treated group were injected GdCl 3 by the tail vein to eliminate the Kupffer cell function in the periportal region selectively. LPS at a dose of 20μg/100g body weight was injected to rats of both groups by the portal vein. Zero, 2, 5, 10, 30, 60 minutes after LPS injection, liver samples were obtained and distribution of LPS in Kupffer cells was observed by the immunofluorescent method using a monoclonal antibody specific to LPS with a confocal laser scanning microscope. RESULTS In normal control group, positive reactions to LPS were found in Kupffer cells in the periportal region with the peak at 2 minutes after LPS injection. Kupffer cells in the pericentral region showed the peak at 5 minutes after LPS injection, but its fluorescent intensity to LPS at the peak time in the cytoplasm was significantly lower than that of Kupffer cells in the pericentral region at the peak point. In GdCl 3 treated group, Kupffer cells in the pericentral region showed the peak at 2 minutes after LPS injection, and its fluorescent intensity to LPS showed no significant difference from that of the normal control rats at the peak point. No significant changes of fluorescent intensities to LPS were found in Kupffer cells in the periportal region at various time points after LPS injection in GdCl 3 treated rats. CONCLUSION Kupffer cells in the periportal and pericentral regions showed difference to uptake LPS in the portal vein.

关 键 词：肝细胞 枯否细胞 脂多糖 代谢 大肠杆菌 

分 类 号：R333.4[医药卫生—人体生理学]