消瘿强肌汤对甲状腺机能亢进性肌病骨骼肌线粒体的影响  

Effects of Xiaoyingqiangji Decoction on Mitochondria in Skeletal Muscle of Hyperthyroid Myopathy

作　　者：于向民[1] 闫凤霞[1] 刘友章[2] 潘晓亮[1] 李玲[1] 

机构地区：[1]青岛大学医学院组织胚胎学教研室,山东青岛市266071 [2]广州中医药大学内科,广东广州市510000

出　　处：《中国康复理论与实践》2007年第7期630-631,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：山东省科技发展计划项目(2004GG220518)。

摘　　要：目的观察消瘿强肌汤对甲状腺机能亢进性肌病大鼠骨骼肌线粒体的影响,探讨消瘿强肌汤治疗甲亢肌病的机理。方法健康雌性Wistar大鼠随机分为4组:甲亢肌病模型组每日腹腔注射甲状腺激素钠盐250μg/100g造模,中药组在造模后灌服消瘿强肌汤30d,生理盐水对照组造模后灌服生理盐水30d,正常对照组每天腹腔注射生理盐水。各组取骨骼肌观察琥珀酸脱氢酶活性和含量的变化,透射电镜观察骨骼肌及线粒体的形态变化。结果甲亢肌病模型组骨骼肌琥珀酸脱氢酶呈色降低,含量下降。超微结构显示线粒体嵴断裂,甚至融合消失。中药组可见酶活性和含量恢复,线粒体嵴排列规则,空泡消失。结论消瘿强肌汤可通过恢复线粒体酶活性和线粒体结构来改善甲亢肌病的骨骼肌功能。Objective To observe the effect of Xiaoyingqiangji decoction on mitochondria in skeletal muscle of hyperthyroid myopathy. Methods Healthy Wistar rats were randomly divided into 4 groups： the group of hyperthyroid myopathy were given L-thyroxine intraperitoneal injection for 42 d; Chinese medicine treated group were treated with Xiaoyingqiangji decoction for 30 d after L- thyroxine injection; the saline control group were given saline solution for 30 d after L-thyroxine injection; normal control group were given saline solution. Then the skeletal muscles were obtained and the succinate dehydrogenase （SDH） were measured. The ultrastructural changes were observed with TEM. Results The activity and content of SDH in the group of hyperthyroid myopathy decreased, and the cristae of mitochondria ruptured and even disappeared. After given Chinese medicine, the activity and content of SDH increased, while the mitochondria showed normal ultrastructural appearance. Conclusion Xiaoyingqiangji decoction can improve the function of skeletal muscles in hyperthyroid myopathy by recovering enzyme activity and mitochondria structure.

关 键 词：甲状腺机能亢进 肌病 消瘿强肌汤 琥珀酸脱氢酶 线粒体 骨骼肌 大鼠 

分 类 号：R581.1[医药卫生—内分泌]