GPx-1基因Pro198Leu多态与2型糖尿病及合并冠心病的关系  

Relationship between Pro198Leu polymorphism of GPx-1 gene and type 2 diabetes mellitus and diabetic coronary heart disease

作　　者：周文锐[1] 刘丽梅[1] 郑泰山[1] 魏盟[2] 

机构地区：[1]上海交通大学第六人民医院内分泌代谢科上海市糖尿病临床医学中心上海市糖尿病研究所,上海200233 [2]上海交通大学第六人民医院心血管内科,上海200233

出　　处：《上海交通大学学报（医学版）》2007年第7期770-773,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(39900071)~~

摘　　要：目的探讨谷胱苷肽过氧化物酶基因(GPx-1基因)Pro198Leu多态性与2型糖尿病(T2DM)及T2DM合并冠心病(CHD)的关系。方法以上海地区汉族人群中的198例T2DM患者和94名口服糖耐量试验(OGTT)正常的非DM对照者作为研究对象。其中T2DM组又分为无冠心病组(n=95)和冠心病组(n=103)。采用PCR扩增-直接测序法,分别检测各组GPx-1基因外显子2的Pro198Leu多态的基因型,并分析比较组间基因型、等位基因频率分布及临床变量间的差异。结果非DM对照组中,GPx-1基因Pro198Leu多态基因型以CC型、等位基因以C型为主,频率分布与文献报道中的瑞典人相比有显著差异(P=0.001);该多态基因型及等位基因频率分布,在无冠心病T2DM组与冠心病T2DM组间比较均无显著性差异(P>0.05);但与非DM对照组相比,T2DM组该多态CT基因型频率显著增加(P=0.012,OR=2.254)。结论上海地区汉族人群中,GPx-1基因Pro198Leu多态CT基因型个体发生T2DM的风险显著增加,但T等位基因并非T2DM发生冠心病的风险因素。Objective To explore the relationship between Pro198Leu polymorphism of glutathione peroxidase-1 （GPx-1） gene and type 2 diabetes mellitus （T2DM） and diabetic coronary heart disease （CHD）. Methods Two hundred and ninety-two subjects in Han Chinese of Shanghai were divided into T2DM （n = 198） group and OGTT normal non-DM controls （n = 94）. The T2DM group was subdivided into non-CHD （n =95） and CHD （n = 103） group, respectively. The individual genotypes of Pro198Leu polymorphism in GPx-1 gene were determined by PCR-direct sequencing. Genotypic and allelic frequencies and clinical characteristics were compared among the groups. Results The most frequent genotype and allele of Pro198Leu polymorphism in GPX-1 gene in non-DM control group were CC and C in Chinese. Significant differences of genotypic and allelic frequencies in the polymorphism were observed between Chinese and Swedish populations （P =0.001）. There were no significant differences in genotypic or allelic frequencies between CHD and non-CHD subgroups （P 〉0.05）. Genotype CT frequency in T2DM group was significantly increased when compared with non-DM control group （ P =0. 012, OR = 2. 254）. Conclusion Pro198Leu polymorphism of GPX-1 gene increases the risk of type 2 diabetes in Han Chinese of Shanghai, while T allele is not a risk factor of diabetic CHD.

关 键 词：2型糖尿病 冠心病 谷胱苷肽过氧化物酶基因 Pro198Leu多态 

分 类 号：R587.1[医药卫生—内分泌]