PAI-1基因多态与中国人2型糖尿病肾病的相关性  

Correlation of plasminogen activator inhibitor-1 gene polymorphism with type 2 diabetic nephropathy in Chinese

作　　者：郑泰山[1] 刘丽梅[1] 周文锐[1] 李鸣[1] 徐靖[1] 陆惠娟[1] 项坤三[1] 

机构地区：[1]上海交通大学第六人民医院内分泌代谢科上海市糖尿病临床医学中心上海市糖尿病研究所,上海200233

出　　处：《上海交通大学学报（医学版）》2007年第7期774-776,共3页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(39900071)~~

摘　　要：目的探讨纤溶酶原激活物抑制因子-1(PAI-1)基因多态与上海地区中国人2型糖尿病肾病的相关性。方法选取2型糖尿病(T2DM)患者247例组成T2DM组,其进一步分为糖尿病无肾病组(n=80)和糖尿病肾病组(n=167),后者又分为微量蛋白尿肾病组(n=129)和显著蛋白尿肾病组(n=38);同时设立非糖尿病对照组(n=87)。应用等位基因特异性PCR法(ASP法)检测PAI-1基因启动子区——675 bp处的4G/5G多态,确定各组PAI-1基因的基因型。采用χ2检验对组间各基因型频率及等位基因频率进行比较,并比较组间携带纯合子风险等位基因4G/4G的基因型频率差异。结果T2DM组与非糖尿病对照组相比,4G/4G基因型及4G等位基因频率无统计学差异(P>0.05)。与糖尿病无肾病组相比,糖尿病肾病组4G/4G基因型频率显著增加(P<0.01,OR=2.4,95%C I=1.3～4.4),4G等位基因频率亦显著增加(P<0.05,OR=1.6,95%C I=1.1～2.3)。结论PAI-1基因多态与中国人T2DM无肾病患者进展为糖尿病肾病显著相关;携带PAI-1基因的4G/4G纯合子基因型的糖尿病患者,其进展为糖尿病蛋白尿肾病的风险增加2.4倍。Objective To explore the relationship between 4G/5G polymorphism of plasminogen activator inhibitor-1 （PAI-1） gene and type 2 diabetic nephropathy （DN） in Chinese Han. Methods Patients with T2DM （n = 247） and non-DM controls （n = 87） were selected for the study. Those with T2DM were divided into non-DN group （n = 80） and DN group （n = 167） , and the latter were sub- divided into microalbuminuria DN group（ n = 129） and predominant proteinuria DN group （ n = 38）. The individual genotypes of 4G/5G polymorphism in PAI-1 gene were determined by allele-specific PCR. Genotypic and allelic frequencies and clinical characteristics were compared among groups by chi square test. Results There was no significant difference in genotypic or allelic frequencies between non- DM control and T2DM groups （P〉0.05）. Compared with non-DN T2DM group, frequency of genotype 4G/4G and allele 4G in DN T2DM group was significantly elevated（P〈0.01, OR =2.4, 95%CI =1.3 -4.4; P〈0.05, OR = 1.6, 95%CI =1. 1 -2.3）. Conclusion 4G/5G polymorphism of PAI-1 gene is well associated with the susceptibility of type 2 diabetic nephropathy, and the 4G/4G homozygote carrier is increased the risk in the development of DN in Chinese subjects with non-DN T2DM.

关 键 词：2型糖尿病 糖尿病肾病 纤溶酶原激活物抑制因子-1 4G/5G多态 

分 类 号：R587.2[医药卫生—内分泌]