地黄活性成分梓醇抗Aβ_(25-35)诱导的PC12细胞凋亡作用  

Effect of catalpol from Radix Rehmanniae on Aβ_(25-35)-induced apoptosis of PC12 cells

作　　者：王金红[1] 张瑞[1] 胡雅儿[1] 夏宗勤[1] 

机构地区：[1]上海交通大学基础医学院细胞调控研究室,上海200025

出　　处：《上海交通大学学报（医学版）》2007年第7期805-808,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30371636)~~

摘　　要：目的探讨地黄活性成分梓醇对β淀粉样蛋白(Aβ25-35)诱导PC12细胞凋亡的保护作用。方法常规培养PC12细胞,加入不同浓度梓醇,24 h后加20μmol/L Aβ25-35,48 h后观察梓醇的作用,MTT法测定细胞存活率,TUNEL法测定细胞凋亡发生率,半定量RT-PCR检测细胞Bax和Bcl-2 mRNA表达。结果梓醇终浓度1×10-5mol/L和1×10-4mol/L显著提高PC12细胞存活率(P<0.05和P<0.01);1×10-4mol/L显著降低细胞凋亡发生率(P<0.01)。Aβ25-35损伤可引起凋亡相关蛋白BaxmRNA的表达增加,梓醇终浓度5×10-5mol/L和1×10-4mol/L有降低作用(P<0.05和P<0.01);同时Aβ25-35使Bcl-2 mRNA的表达降低,梓醇有提高作用(P<0.05和P<0.01)。结论地黄活性成分梓醇有对抗Aβ25-35损伤引起的PC12细胞凋亡作用。Objective To investigate the effect of catalpol from Radix Rehmanniae on Aβ25-35-induced apoptosis of PC12 cells. Methods PC12 cells were routinely cultivated and treated by Aβ25-35 （ final concentration, 20 μmol/L） 24 hours after the addition of catalpol or saline. Forty-eight hours later, cells were examined for viability and apoptosis by MTT method and TUNEL method, respectively, while Bax and Bcl-2 mRNA expression were analyzed by semi-quantitive RT-PCR. Results Catalpol could significantly elevate the viability at 1 ×10^-5 mol/L and 1 ×10^-4 mol/L （P 〈 0.05 and P 〈 0.01, respectively）and decrease the apoptotic rate at 1 ×10^-4 mol/L of PC12 cells induced by Aβ25-35 （P 〈0.01 ）. The expression of Bax mRNA was higher and Bcl-2 mRNA was lower in cells injured by Aβ25-35 than the control, while catalpol at final concentrations of 5 ×10^-5 mol/L and 1 ×10^-4 mol/L could suppress the elevation of the former （P 〈0.05 and P 〈 0.01, respectively） and inhibit the drop of the latter（ P 〈 0.05 and P 〈 0.01, respectively）. Conclusion Catalpol, the active component of Radix Rehmanniae, has antiapoptotic effect on PC12 cells induced by Aβ25-35.

关 键 词：地黄活性成分 梓醇 PC12细胞 细胞凋亡 AΒ25-35 

分 类 号：R285[医药卫生—中药学]