小肠黏膜下层复合雪旺细胞粗制品桥接周围神经缺损  

Graft repair of peripheral nerve defects using small intestinal submucosa with crude Schwann cells

作　　者：谢雪涛[1] 张长青[1] 苏琰[1] 

机构地区：[1]上海交通大学第六人民医院骨科,上海200233

出　　处：《上海交通大学学报（医学版）》2007年第7期814-817,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30371444)~~

摘　　要：目的观察小肠黏膜下层(SIS)复合雪旺细胞粗制品修复周围神经缺损的效果。方法选取健康雄性SD大鼠36只,随机平均分为3组(n=12)。致大鼠坐骨神经12 mm缺损后,分别用SIS桥接修复(A组),SIS复合雪旺细胞粗制品修复(B组)和自体神经移植修复(C组)。术后16周取材,通过组织学观察、肌肉湿重测量、计算机图像分析、Trueb lue逆行示踪和透射电镜观察来评价神经再生效果。结果组织学观察、Trueb lue逆行示踪和透射电镜观察证实三组均有再生神经纤维通过缺损区;B组和C组大鼠的小腿三头肌湿重、单位面积轴突数量和神经组织面积均显著优于A组(P<0.05),B、C两组间的差异则无统计学意义(P>0.05)。结论SIS复合雪旺细胞粗制品修复周围神经缺损可达到自体神经移植的效果,有望替代自体神经移植。Objective To investigate the results of graft repair of peripheral nerve defects using small intestinal submucosa （SIS） with crude Schwann cells. Methods Thirty-six male SD rats were randomly divided into three groups, with 12 in each group. Firstly, 12-mm gaps of sciatic nerve were made in rats and then treated respectively with porcine SIS （ group A） , SIS compounded with crude Schwann cells （group B） and auto-nerve grafting （group C） in the three groups. The grafts were harvested 16 weeks postoperatively to evaluate the results of nerve regeneration through histological examination, triceps surae weight, computerized imaging analysis, Trueblue retrograde tracing and transmission electron microscopy. Results Nerve regeneration was confirmed to have extended through the gaps according to the results of histological examination, Trueblue retrograde tracing and transmission electron microscopy. Furthermore, in the respect of triceps surae weight, quantity of axis cylinder per area and percentage of neural tissue, the results of group B and group C were superior to those of group A with a significantly statistical difference （ P 〈 0.05） ; whereas no significant difference was found between group B and group C （P 〉0.05）. Conclusion SIS compounded crude Schwann cells was able to achieve the outcome of auto-nerve grafting and was a promising replacement graft.

关 键 词：小肠黏膜下层 雪旺细胞 周围神经缺损 神经导管 大鼠 

分 类 号：R651.3[医药卫生—外科学]