果糖饮水对Ⅱ型糖尿病ICR小鼠体重和血糖的影响  

Effect of Fructose on Body Weight and Plasma Glucose in Type Ⅱ Diabetes Mellitus ICR Mice Induced by High Fat Diet and Streptozotocin

作　　者：牛屹东[1] 王新生[1] 

机构地区：[1]北京大学人民医院实验动物中心,北京100044

出　　处：《实验动物科学》2007年第3期1-5,共5页Laboratory Animal Science

基　　金：北京大学人民医院研究与发展基金资助(P886)

摘　　要：目的观察果糖饮水对高脂饲料-STZ诱导ICR小鼠Ⅱ型糖尿病模型过程中体重及血糖浓度变化的影响。方法选取ICR小鼠(3周龄)雌雄各半,先以高脂饲料及高脂饲料加3%果糖饮水喂养6周,再以链脲佐菌素(STZ)(100 mg/kg体重)腹腔注射,继续喂养6周。每周测定体重,于注射前及注射后4周测定非空腹血糖浓度。结果雄性高脂饲料-果糖饮水组和高脂饲料组体重显著高于对照组(P<0.05),而高脂饲料-果糖饮水组与高脂饲料喂养组间没有显著差异(P>0.05);雌性高脂饲料-果糖饮水组和高脂饲料组体重与对照组均无显著差异(P>0.05),但高脂饲料-果糖饮水组体重显著低于高脂饲料组(P<0.05)。喂养6周后(注射STZ前),高脂饲料-果糖饮水组和高脂饲料组的血糖浓度均显著高于对照组(P<0.05),而实验组间没有显著差异。注射STZ后,雄性高脂饲料-果糖饮水组和高脂饲料组的血糖浓度均显著高于对照组(P<0.01),并且都超过糖尿病小鼠非空腹血糖的成模标准(11 mmol/L),且高脂饲料-果糖饮水组血糖浓度又显著高于高脂饲料喂养组(P<0.05);雌性小鼠高脂饲料-果糖饮水组和高脂饲料组血糖浓度均显著高于对照组(P<0.01),但都没有达到糖尿病小鼠非空腹血糖的成模标准,且高脂饲料-果糖饮水组与高脂饲料组间血糖浓度没有显著差异(P>0.05)。结论在高脂饲料喂养联合腹腔注射STZ制备Ⅱ型糖尿病模型实验中,果糖饮水对高脂饲料致肥胖作用没有显著的改善,而对血糖浓度升高具有明显的促进作用。这种促进作用在雄性小鼠最为明显,而对雌性小鼠没有明显效果。Objective To investigate the effect of fructose on body weight and non-fasting plasma glucose of type Ⅱ diabetes mellitus ICR mice induced by high fat diet and streptozotocin. Methods Twenty ICR mice （21 d） were exposed to high fat diet （HFD）, high fat diet-3% fructose （HFDF） and standard rodent chow, respectively. After exposure to the diets for 6 weeks, mice were injected intraperitoneally with either STZ 100 mg/kg body weight or vehicle （0. ! mol/L citric acid buffer, pH 4.5） and kept on the same diet for the next 6 weeks. Body weight was recorded weekly, and non-fasting blood glucose was measured before and at 1 ～ 4 weeks post STZ. Results The body weights of HFD-fed and HFDF-fed male mice were significantly higher than those of the chow-fed mice （ P 〈 0.05）, but there was no significant difference in body weight between HFD-fed and HFDF-fed male mice（ P 〉 0.05） ; body weights of HFDF- fed female mice were significantly lower than those of the HFD-fed mice（ P 〈 0.05）, and there was no obvious difference in body weights between HFD-fed and chow-fed female mice or between HFDF-fed and chow-fed female mice （ P 〉 0.05）. The blood glucoses of HFD-fed and HFDF-fed mice were statistically higher than those of chow-fed mice （ P 〈 0.01 ） after exposure to the respective diets for 6 weeks, in both sex, but there was no difference between HFD-fed and HFDF-fed mice. Both blood glucoses of HFD-fed and HFDF-fed male mice were greater than 11 mmol/L （standard value for non-fasting blood glucose of diabetes mellitus model in mice ）, whereas it was not observed in female mice. Conclusion HFDF-feeding improves increasing of non-fasting blood glucose significantly than HFD-diet in male mice, but it does not improve the development of obesity in diabetes mellitus ICR mice induced by HFD-STZ in both sex. HFDF-feeding is not beneficial to developing type Ⅱ diabetes mellitus model in ICR mice because the concentration of plasma glucose increases too fast.

关 键 词：果糖 高脂饲料 链脲佐菌素 ICR小鼠 糖尿病 

分 类 号：R965.1[医药卫生—药理学]