中华眼镜蛇毒组分H对大鼠缺血再灌注心肌细胞凋亡及相关凋亡基因表达的影响  

Effect of fraction H of Naja naja Venom on cardiomyocyte apoptosis and gene expression after ischemia-reperfusion in rats

作　　者：江中喜[1] 侯力强[2] 

机构地区：[1]广州医学院第二附属医院心内科,广州市510260 [2]广州医学院蛇毒研究所,广州市510182

出　　处：《广西医学》2007年第6期794-796,共3页Guangxi Medical Journal

基　　金：广州市医药卫生科研课题(2005-YB-136)

摘　　要：目的观察中华眼镜蛇毒组分H预防性给药对大鼠缺血再灌注心肌细胞凋亡及相关凋亡基因Bcl-2和Bax表达的影响。方法30只SD大鼠随机分为对照组、缺血再灌注组、组分H组各10只。采用原位末端标记(TUNEL)法标记凋亡心肌细胞,免疫组化法测缺血再灌注心肌细胞Bcl-2及Bax的表达。结果心肌TUNEL阳性指数组分H组为(26.2±3.5)%,缺血再灌注组为(34.6±5.3)%,对照组为(3.4±1.8)%,3组比较差异有统计学意义(P<0.01),尤以缺血再灌注组更明显。缺血再灌注组Bcl-2表达降低(0.113±0.02),与对照组(0.146±0.05)比较差异有统计学意义(P<0.01);组分H组Bcl-2表达(0.128±0.03)与缺血再灌注组比较显著提高(P<0.01),但仍低于对照组(P<0.01)。缺血再灌注组Bax表达升高(0.105±0.03),与对照组(0.070±0.01)比较差异有统计学意义(P<0.01);组分H组Bax表达(0.084±0.02)虽比缺血再灌注组降低(P<0.01),但仍高于对照组(P<0.01)。结论中华眼镜蛇毒组分H有显著抗缺血再灌注心肌细胞凋亡作用,调控心肌细胞凋亡基因Bcl-2及Bax蛋白表达可能是其重要机制之一。Objectives To study the effect of prophylactic admistration of fraction H of Naja naja Venom on ischernia-reperfusion rats,and the influence on the expression of cardiomyocyte apoptosis and the gene Bcl-2 and Bax. Methods Thirty adult SD rats were divided into three groups ,fraction H of Naja naja Venom group,ischemia-reperfusion group and control group. The expression of apoptotic cardiomyocyte was determined by Tunel staining. Immunohistochemistry was used to detect mRNA the expression of Bcl-2 and Bax in myocardial cells of rats with ischemia reperfu- sion. Result The apoptotic index was significantly increased in both fraction H of Naja naja Venom group（26.2±3.5）% and ischemia-repeffusion group （34.6±5.3）% （P 〈0. 01） ,especially in fraction H of Naja naja Venom group. The expression of Bcl-2 was decreased significantly in ischemia-reperfusion group than in control group （0. 113±0.02 VS 0. 146 ±0.（15） （P 〈0.01） ,the expression of Bcl-2 in fraction H of Naja naja Venom group was higher than ischemia-reperfusion group, but still lower than control group （P 〈 0. 01 ）. The expression of Bax significantly in ischemia-reperfusion group than in control group （0. 105 ±0.03 VS 0.070 ±0. 01） （P 〈0.01 ） ,the expression of Bax in fraction H of Naja naja Venom group was lowerr than ischemia-reperfusion group,but shill lower than control group （P〈0.01）. Conclusion Fraction H of Naja naja Venom may effectively protect cardiomyocyte apoptosis induced by ischemia-reperfusion,and one of the mechanisms of it may be adjust the expression of protein Bcl-2 and Bax.

关 键 词：冠心病 中华眼镜蛇毒 组分H 缺血再灌注 细胞凋亡 基因表达 

分 类 号：R541.4[医药卫生—心血管疾病]