细菌伴侣蛋白Skp引起白细胞趋化运动的体内实验研究  

Leukocyte infiltration induction capacity of Skp in vivo

作　　者：施磊[1] 冯影 

机构地区：[1]中国医科大学附属第一医院耳鼻喉科,辽宁沈阳110001 [2]辽阳市中心医院耳鼻喉科,辽宁辽阳111000

出　　处：《中国现代医学杂志》2007年第13期1599-1602,共4页China Journal of Modern Medicine

基　　金：教育部留学回国人员科研启动基金(教外司留[2005]546);辽宁省博士启动基金(20051048)

摘　　要：目的细菌伴侣蛋白(Seventeen-Kilodalton protein,Skp)可以作用于C5a受体引起白细胞的趋化作用。该研究的目的Skp在生物体内和体外实验一样可以引起白细胞的趋化运动,且是细菌提取掖中主要的白细胞趋化因子。方法制备Skp的特异性抗体,进行免疫吸收实验,进而豚鼠体内实验,观察组织标本。结果E.Coli提取液中主要的白细胞趋化活性来自于Skp。Skp提纯物注射后可在豚鼠皮内发生单核细胞和PMNs的大量浸润。结论细菌体内的Skp可能与在细菌壁发生补体系统激活产生的C5a共同引起白细胞的趋化反应。对于革兰氏阴性病原菌的先天性免疫具有重要意义。[Objective] Skp-an Escherichia coli periplasmic protein induces the chemotactic responses of monocytes and polymorphonuclear leukocytes via C5a receptor. We examined the capacity of Skp to induce leukocyte infiltration in vivo. [Methods] preparation of monospecific antibodies against Skp, using in immunoabsorption experiments, and Histological examination. [Results] Both experiments indicated Skp is the major leukocyte chemotactic factor in the E.coli extract and Skp possesses the capacity to recruit PMNs and monocytes to extravascular tissue space from the blood circulation. [Conclusion] Skp leaked from the bacteria results in a steadier leukocyte chemotactic response, would be important for phagocytic leukocytes playing roles in the innate immunity, at least against gram-negative bacteria,

关 键 词：Skio C5A C5A受体 白细胞趋化运动 

分 类 号：R392.12[医药卫生—免疫学]