人CtBP2与CCNH相互作用的初步研究  

Preliminary Research into the Interaction between Human CtBP2 and CCNH

作　　者：马雅婷[1] 赵晶[1] 杨光[1] 王玮[1] 施慧莉[1] 霍克克[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《复旦学报（自然科学版）》2007年第3期394-400,共7页Journal of Fudan University：Natural Science

基　　金："863"人类肝脏蛋白质相互作用研究项目(2006AA02A310);上海市科技创新团队项目(03DZ14024)

摘　　要：CtBP2(E1AC-terminal binding protein 2)作为辅阻遏物与多种转录因子联系而参与到很多生物过程中,如细胞分化、凋亡、发育和肿瘤发生等,然而其中许多作用机制尚不明了.为了对CtBP2进行深入研究,利用高通量酵母双杂交技术,以人CtBP2为诱饵,与含有1000个人肝基因克隆的酵母双杂交文库进行接合筛选获得了一个与它相互作用的猎物蛋白CCNH(Cyclin H).通过GST-pull down、免疫共沉淀和亚细胞共定位等实验进一步证明了这两个蛋白在体外和体内的相互作用.CtBP2（E1A C-temainal binding protein 2） has linked to multiple biological precesses through their association with numerous transcription factors. It plays a crucial role in differentiation, apoptosis, development and oncogenesis. However, how it represses the transcription is not clear. For further study, the high through-put yeast two hybrid system was used to do large scale mating with the library of 1000 yeast clones containing genes of human liver proteins by using Human CtBP2 as a bait. CCNH which interacts with CtBP2 was identified. Pull-down and coimmunoprecipitation were used to confirm this interaction. The co-loclization assay showed that CtBP2 co-lccalizes with CCNH in the nucleus.

关 键 词：E1A C末端结合蛋白2 细胞周期蛋白H 酵母双杂交 蛋白质-蛋白质相互作用 

分 类 号：Q71[生物学—分子生物学]