实时荧光定量PCR检测原发性肝癌中hNTKL-BP1基因的表达  

Detection of hNTKL-BP1 Gene Expression in Primary HCC by Using RFQ-PCR

作　　者：严昕[1] 王均永[1] 吴小末[1] 施慧莉[1] 霍克克[1] 

机构地区：[1]复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《复旦学报（自然科学版）》2007年第3期411-416,420,共7页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金资助项目(39980020);"863"中国人肝蛋白质组研究项目(2004BA711A19);"863"人类肝脏蛋白质相互作用研究项目(2006AA02A310);上海市科技创新团队项目(03DZ14024)

摘　　要：分别提取手术切除人原发性HCC肝癌和癌旁肝组织总RNA并逆转录为cDNA,在hNTKL-BP1基因的高保守区设计相应引物和Taqman荧光探针,实时检测PCR产物的荧光强度,采用2-△△Ct方法分析比较该基因在人类肝癌组织和癌旁肝组织中的表达差异.结果是44例原发性HCC肝癌组织中hNTKL-BP1基因表达水平显著高于癌旁肝组织(t=2.69,P<0.01),肝癌组织与癌旁肝组织hNTKL-BP1基因表达差异和肿瘤大小有一定的相关性(P=0.028),hNTKL-BP1基因可能与肝癌的发生、发展有一定相关性.The total RNA isolated from surgical specimens of HOC and adjacent non-cancerous liver tissues were obtained from 44 patients with HOC who underwent partial hepatectomy, followed by reverse transcribed into cDNA. Specific primers and TaqMan probes of hNTKL-BP1 were used in RFQ-PCR. The products were detected continuously during the amplification process. The expression levels of target gene in tumor tissues and adjacent liver tissues were compared by 2 - △△Ct method. The results from 44 specimens showed that the expression level of hNTKL-BP1 in primary HCC rumor tissues was significantly higher than that in adjacent non-cancerous liver tissues （ t = 2.69, P 〈 0.01） and the positive correlation between expression difference and tumor size was indicated （ P = 0. 028）. It is therefore poasble that hNTKL-BP1 may be associated with the development, progress of human HCC.

关 键 词：N端激酶结构样蛋白结合蛋白 原发性肝细胞癌 实时荧光定量PCR 

分 类 号：R3[医药卫生—基础医学]