重组戊型肝炎病毒衣壳蛋白p239特异性吸附、侵入HepG2细胞并阻断野病毒对肝细胞的感染  

Recombinant HEV Caspid Protein p239 Specifically Attached on HepG2 Cells and Blocked the Infection of Wild-type HEV on Liver Cells

作　　者：苗季[1] 郑子峥[1] 何水珍[1] 刘平果[2] 吴小成[1] 孙媛媛[1] 唐明[1] 张军[1] 夏宁邵[1] 

机构地区：[1]国家传染病诊断试剂与疫苗工程技术研究中心厦门大学生命科学院,福建厦门361005 [2]厦门大学附属中山医院肝胆外科,福建厦门361004

出　　处：《病毒学报》2007年第4期331-334,共4页Chinese Journal of Virology

基　　金：福建省科技重大专项项目(2004YZ01);福建省青年科技人才创新项目(2006F3124)

摘　　要：By using Western blot and immunofluorescence assays,the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature.Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells,indicating that p239 and HEV competed the same targeting site on these cells.These data provide evidence that p239 has a similar cell surface structure with wild-type HEV.By using Western blot and immunofluorescence assays, the recombinant HEV capsid protein p239 was found specifically attached to the HepG2 cell surface and entered to the cytoplasm with the increase of incubation temperature. Pre-mixture of wild-type HEV with p239 blocked the infectivity of the virus on primary cultured human hepatocytes and HepG2 cells, indicating that p239 and HEV competed the same targeting site on these cells. These data provide evidence that p239 has a similar cell surface structure with wild-type HEV.

关 键 词：戊型肝炎病毒(HEV) 病毒吸附 

分 类 号：R373.2[医药卫生—病原生物学]