青霉素高产菌株的理性筛选  

Rational screening of penicillin high producing strain

作　　者：娄忻[1] 张莉[1] 刘靓[1] 张玉莲[1] 陈丽华[1] 刘静[1] 贺建功[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心,石家庄050015

出　　处：《中国抗生素杂志》2007年第7期403-404,446,共3页Chinese Journal of Antibiotics

摘　　要：以青霉素生产菌种87117#为出发菌种,采用紫外线诱变复合前体动态后处理的方法,结合限氧条件下的摇瓶筛选,从177支前体抗性株中筛出XY-137#高产突变株。该菌株摇瓶效价平均提高了8.8%,且遗传特性稳定,单位菌丝产物合成能力强。XY-137#菌株在百吨罐中经过近百批的生产验证,其放罐效价、发酵指数、罐批产量平均提高了3.83%、5.66%和2.69%。Using Penicillium chrysogenum # 87117 as starting strain, a penicillin high yield strain #XY-137 was obtained by UV irradiation combined with precursor-resistant strain in shake flask selection method under limited oxygen. Penicillin potency of strain #XY-137 was increased by 8.8% cultured in shaking flasks. Average productivity of about 100 batches fermentation level was increased by 2.69%-5.66% than that of strain #87117 in 100-ton fermentor.

关 键 词：紫外诱变 动态后处理 理性筛选 青霉素高产菌株 

分 类 号：TQ465.1[化学工程—制药化工]