黄芪对哮喘大鼠神经内分泌免疫网络相关指标的影响  

Effects of Milkvetch Root on Neuro-endocrino-immune Network in Asthma Model Rat

作　　者：董竞成[1] 赵福东[1] 谢瑾玉[1] 崔焱[1] 刘宝君[1] 宫兆华[1] 巫善美[1] 

机构地区：[1]复旦大学附属华山医院中西医结合肺,炎症和肿瘤研究室,复旦大学上海医学院中西医结合系,上海200040

出　　处：《中国中西医结合杂志》2007年第7期619-622,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：上海市科委重点资助项目(No.054119648)

摘　　要：目的观察益气药黄芪对支气管哮喘(以下简称哮喘)反复发作模型大鼠神经内分泌免疫(NEI)网络相关指标的影响。方法取健康清洁级雄性挪威大鼠54只,分为正常组、模型组、黄芪低、中、高剂量组。除正常组外,其他4组均行哮喘造模,黄芪不同剂量组分别灌胃给予相应剂量的黄芪,模型组给予等量生理盐水。用Realtime-PCR法检测下丘脑促肾上腺皮质激素释放激素(CRH)mRNA的表达;放免法检测血浆中促肾上腺皮质激素(ACTH)的含量;酶联免疫吸附(ELISA)法检测血清细胞因子(IL-4、IL-6、IFN-γ)和血清皮质酮(CORT)的含量;肺组织采用HE染色观察其病理变化。结果各组大鼠下丘脑CRHmRNA表达和血清ACTH无明显变化,造模后各组大鼠血清CORT水平升高(P<0.05);黄芪低组大鼠血清CORT含量显著高于其他各组(P<0.05或P<0.01);黄芪高组血清IL-4和IL-6含量显著下降(P<0.01),且有量效关系;IFN-γ各组间比较差异无显著性。各组大鼠肺组织病理变化比较差异无显著性。结论哮喘大鼠存在NEI网络相关指标的紊乱;黄芪可以增强下丘脑-垂体-肾上腺皮质轴的功能,改善Th1和Th2型细胞因子的平衡,从而调节了NEI网络的紊乱。Objective To study the change and the effect of milkvetch root （MR） on neuro-endocrino-immune （NEI） network related indexes in repeated asthmatic attack model rats. Methods Rats were randomly divided into five groups： the normal group （A）, the model group （B）, and the three treated groups （C, D, E） treated with low, medium and high dose of the MR by gastrogavage. The corticotropin releasing hormone （CRH） mRNA expression in hypothalamus was tested by Realtime-PCR, serum adrenocorticotropic hormone （ACTH） and corticosterone （CORT） were detected with radioimmunoassay, serum IL-6, IL-4, IFN-γ determined with enzyme-linked immunosorbent assay, and the lung tissue pathology was examined by hematoxylin and eosin staining. Results As compared with the normal group, in the rats 3 weeks after modeling, CRH mRNA expression, blood IFN-γand plasma ACTH were unchanged, serum level of CORT raised significantly （P〈0.05）, IL-6 and IL-4 showed an increasing trend but without significance. Low dose of MR could promote the production of serum CORT, and high dose of MR could down-regulate the concentrations of IL-4 and IL-6 （P 〈 0.01 ）. No significant difference was found in comparison of pathological changes of lung tissue among the groups. Conclusion Rats suffered from repeated asthmatic attack have some disorders in indexes of NEI, MR could enhance the function of hypothalamic-pituitary-adrenal cortex axis and adjust the balance of Thl and Th2 cytokines to alleviate the inflammation of asthma.

关 键 词：黄芪 支气管哮喘 炎症 神经内分泌免疫网络 

分 类 号：R285.5[医药卫生—中药学]