川芎嗪对帕金森病大鼠脑内灌流左旋多巴引起的脑氧化损伤的作用  

Effects of Tetramethylpyrazine on Brain Oxidative Damage Induced by Intracerebral Perfusion of L-DOPA in Rats with Parkinson's Disease

作　　者：王丹巧[1] 王巍[1] 景富春[2] 赵晋宁[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091 [2]新疆石河子大学药学院

出　　处：《中国中西医结合杂志》2007年第7期629-632,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局资助项目(No.04-05ZP65)

摘　　要：目的探讨川芎嗪对左旋多巴(L-DOPA)脑内灌流引起的帕金森病(PD)大鼠脑氧化损伤的影响。方法用6-羟基多巴胺(6-OHDA)脑内注射造成PD大鼠模型;采用微透析技术脑内灌流L-DOPA和水杨酸捕获羟自由基,高效液相-电化学检测,动态观察大鼠纹状体细胞外液羟自由基被捕获所形成的2,3-二羟基苯甲酸(2,3-DHBA)、2,5-二羟基苯甲酸(2,5-DHBA)浓度的变化。结果模型组大鼠2,3-DHBA和2,5-DHBA浓度较假手术组分别出现6和7个时间点的显著升高(P<0.05或P<0.01);与模型组比较,川芎嗪大、小剂量组在多个时间点其水平明显降低(P<0.05或P<0.01)。结论川芎嗪可减轻L-DOPA引起的PD大鼠脑氧化损伤。Objective To observe the effects of tetramethylpyrazine （TMP） on brain oxidative damage induced by intracerebral perfusion of levodopa （L-DOPA） in rats with Parkinson＇s disease （PD）. Methods PD model rats were induced by intracerebral injection of 6-hydroxyl dopamine （6-OHDA） and perfused in brain with L-DOPA using microdialysis technique. Changes in levels of 2,3-dihydroxy benzyl acid （2,3-DHBA） and 2,5- dihydroxy benzyl acid （2,5-DHBA） in striatum of rats, formed by extracellular hydroxyl radical from salicylic acid capturing, were dynamically observed at various time points by HPLC-ED. Results After treatment with L-DOPA, 2,3-DHBA and 2,5-DHBA in the model group showed significantly higher levels at 6 and 7 time points as compared with those in the sham-operated group at the corresponding time points （ P 〈 0.05 or P 〈 0.01 ）, while these abnormal elevations were significantly inhibited in the TMP treated groups, either in large or small dose （P〈 0.05 or P 〈 0.01 ）. Conclusion TMP could reduce the L-DOPA induced brain oxidative damage in PD rats.

关 键 词：川芎嗪 左旋多巴 羟自由基 帕金森病 脑内微透析 

分 类 号：R285.5[医药卫生—中药学]