ICAM-1在小鼠到大鼠心脏移植中的表达及来氟米特与环孢素A对其表达的影响  

The expression of ICAM-1 in mouse to rat cardiac xenograft and the effects of leflunomide and cyclosporine

作　　者：杨光伦[1] 姚榛祥[1] 黄平[1] 涂刚[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,400016

出　　处：《中华器官移植杂志》2007年第7期400-402,共3页Chinese Journal of Organ Transplantation

基　　金：重庆市卫生局科研课题资助(05-2-180)

摘　　要：目的探讨细胞间粘附分子1(ICAM-1)在小鼠到大鼠异种心脏移植中的表达,以及来氟米特(Lef)和环孢素A(CsA)对其表达的影响。方法以NIH小鼠为供者,Wistar大鼠为受者,施行异位(颈部)心脏移植,实验分4组进行,CsA组受者术后接受CsA腹腔注射,Lef组受者术后接受Lef灌胃,联合用药组受者术后接受Lef和CsA治疗,另设空白对照组,受者术后不进行任何处理。以移植心脏停跳作为排斥反应的终点,采用免疫组化和蛋白印迹杂交法检测移植心肌组织中ICAM- 1蛋白的表达。结果空白对照组、CsA组、Lef组及联合用药组移植心的存活时间分别为(2.17±0.41)d、(2.50±1.05)d、(4.17±1.33)d和(6.50±2.56)d,联合用药组明显长于其它三组(P<0.05),Lef组明显长于空白对照组(P<0.05)。移植心脏组织中ICAM-1蛋白的表达强度(电泳条带积分吸光度值),空白对照组为155.40±5.33,CsA组为150.73±5.13,Lef组为104.65±6.15,联合用药组为29.24±2.76,联合用药组的积分吸光度值明显低于其它三组(P<0.05),Lef组的积分吸光度值明显低于CsA组和空白对照组(P<0.05)。结论小鼠到大鼠的异种心脏移植中ICAM-1表达明显,联合应用Lef和CsA可显著抑制ICAM-1在移植心脏中的表达。Objective To investigated the expression of intracellular adhesion molecule-1 （ICAM-1） in mouse to rat cardiac xenograft and the effects of leflunomide and cyclosporine. Methods NIH mice and Wistar rats served as donors and recipients respectively. Mouse to rat heterotopic heart transplantation was performed （in the neck）. The experiments were divided into 4 groups： CsA group, leflunomide （Lef） group, CsA ＋ Lef group, control group. It was the time of rejection that no pulsation could be detected in the transplanted heart. The expression of ICAM-1 protein in cardiac xenograft tissue was detected by immunohistochemistry and Western blot in each group. Results The survival time of cardiac xenograft in control group, CsA group, Lef group and CsA ＋ Lef group was （2. 17 ±0. 41）, （2. 50 ± 1.05）, （4. 17± 1.33） and （6. 50 ± 2. 56） days respectively. The expression of ICAM-1 protein （IOD） in cardiac xenograft tissue in control group, CsA group, Lef group and CsA＋ Lef group was 155.40 ± 5. 33, 150. 73 ±5.13, 104. 65 ± 6. 15 and 29. 24 ± 2. 76 respectively. Conclusion The expression of ICAM-1 protein was strong in mouse to mt cardiac xenograft. The combined use of Lef and CsA can significantly suppress the expression of ICAM-1 protein in the cardiac xenograft.

关 键 词：移植 异种 心脏移植 胞间粘附分子1 免疫抑制剂 

分 类 号：R654.2[医药卫生—外科学]