A群猪轮状病毒JS株VP6基因序列分析  

Sequence analysis of VP6 gene of group Porcine Rotavirus A JS strain

作　　者：高秀春[1] 时洪艳[2] 佟有恩[2] 吴波平[1] 孙东波[2] 白兴华[2] 陈建飞[2] 冯力[2] 

机构地区：[1]东北农业大学生命科学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室猪传染病研究室,黑龙江哈尔滨150001

出　　处：《黑龙江畜牧兽医》2007年第7期27-29,共3页Heilongjiang Animal Science And veterinary Medicine

摘　　要：参考GenBank中发表PRVOSU毒株VP6序列ORF两端保守区，设计1对特异引物，以PRVJS株反转录cDNA为模板，通过PCR方法获得约1．3kb的基因片段，并克隆到pMD18-T载体中进行序列测定。测序结果表明：获得了VP6的1320bp全基因序列；将其ORF与国内外9株PRVVP6基因的ORF进行核苷酸和氨基酸同源性比较，核苷酸同源性在77．1％～91．1％之间，氨基酸的同源性在89．7％～99．5％之间；在氨基酸系统发育进化树中，JS毒株与A131、OSU、CN86毒株亲缘关系较近．为一个进化群。Based on VP6 sequence of PRV OSU strain published in GenBank, a pair of primer within the conserved region flanked the ORF was designed, and 1.3 kb fragment was amplified by PCR, which was then inserted to the pMD18 -T vector and the sequence was analyzed. The result showed that complete sequence （ 1 320 bp） of JS VP6 gene was obtained. JS VP6 gene has a high sequence homology to those of 9 PRV isolates. The homology of nucleotide sequences and amino acids sequences were 77. 1% - 91.1% and 89.7 % -99.5 %, respectively. JS strains had a closer relationship with A131, OSU, CN86 strains by phylogenetic trees of VP6 of PRV, so they maybe belong to the same group

关 键 词：PRV VP6基因 序列分析 

分 类 号：S852.659.4[农业科学—基础兽医学]