氨己烯酸和阿托品对急性乐果中毒小鼠的保护作用  

Protective effects of vigabatrin and atropine against dimethoate induced-intoxication in mice

作　　者：吴强恩[1] 班婷婷[1] 姚新民[1] 常秀丽[1] 吴庆[1] 周志俊[1] 

机构地区：[1]复旦大学公共卫生学院教育部公共卫生安全重点实验室,上海200032

出　　处：《中华劳动卫生职业病杂志》2007年第7期389-393,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目(30571554);上海市曙光学者计划基金资助课题

摘　　要：目的研究氨己烯酸和阿托品对乐果急性中毒小鼠的保护作用。方法根据正交实验设计原理，选用L9（3^4）正交表，以存活时间、肌束震颤出现时间和翻正反射消失时间为指标，分别测试染毒前1．5h给予0、50和100mg／kg氨己烯酸和（或）染毒后立即给予0．0、2．5和5．0mg／kg阿托品对乐果急性染毒小鼠的保护效果。结果小鼠存活时间拟合模型的方差分析结果中主效应氨己烯酸F氨=4．73（P=0．015），阿托品F阿=50．88（P=0．000），两者的交互作用F氨×阿=4．17（P=0．007），均有统计学意义。各因素的极差比较结果为R阿〉2R氨或2R氨×阿，阿托品和氨己烯酸都可延长中毒小鼠的存活时间，两者存在交互作用。肌束震颤出现时间方差分析F氨=6．87（P=0．003），有统计学意义，但F阿=0．03（P=0．968），F氨×阿=1．134（P=0．356），均无统计学意义。各因素极差进行比较，R氨〉2R阿或2氨×阿。氨己烯酸可延缓乐果中毒小鼠的肌束震颤出现时间，但不能阻止其最终发生；阿托品对肌颤没有保护作用，且两者之间无交互作用。翻正反射统计结果：F氨=5．81（P=0．006），F阿=9．05（P=0．001），均有统计学意义，但F氨×阿=1．34（P=0．257），无统计学意义。各因素极差比较结果：R氨或R阿〉2R氨×阿。阿托品和氨己烯酸均可延长小鼠的翻正反射存在时间，但两者不存在交互作用。结论氨己烯酸可改善乐果中毒小鼠的体征，延长中毒小鼠的存活时间，并与阿托品有一定的协同保护作用。Objective To investigate the protective effects of vigabatrin and atropine against the acute toxicity of dimethoate in male Kun-min mice. Methods The therapeutic schedules of vigabatrin （50 or 100 mg/kg） and （or） atropine（2.5 or 5.0 mg/kg） were performed according to the L9（3^4）orthogonal test table. The survival time, the righting reflex and the onset of muscle fasciculations were observed after the administration of dimethoate. Results First, the main effects of vigabatrin, atropine and the interaction between them were statistically significant in the Univariate analysis of the survival time at the alpha level of 0.05 （FV=4.73, PV= 0.015; FA=50.88, PA=0.000; FV×A=4.17, PV×A=0.007）. The range of atropine was more than double of that of vigabatrin or their interaction （RA〉2RV or 2RV×A）.So not only atropine and vigabatrin but also their combination interaction protected mice against dimethoate lethality. The atropine played the major role in diminishing the lethality induced by dimethoate. Second, only vigabatrin, while not atropine, delayed the mice from dimethoate-induced muscle fasciculation according its statistical results （FV=6.87, PV=0.003; FA=0.03, PA=0.968; FV×A= 1.134, PV×A=0.356）. It should be noted that vigabatrin could not completely prevent dimethoate induced-muscle fasciculation in the test. At last, both atropine and vigabatrin could maintain the righting reflex in the intoxication, however there was no interaction between them （FV=5.81, PV=0.006; FA=9.05, PA=0.001; FV×A=1.34, PV×A= 0.257）. Conclusion Combined treatment with atropine and vigabatrin in the organophosphates intoxication seems reasonable and acceptable.

关 键 词：氨己烯酸 阿托品 Γ-氨基丁酸 乐果 正交试验 

分 类 号：R114[医药卫生—卫生毒理学]