焦炉工人外周血淋巴细胞热休克蛋白72水平及与DNA损伤的关系  

Relationship between heat shock protein 72 and DNA genetic damage in peripheral blood lymphocytes of coke oven workers

作　　者：郑金平[1] 孙建娅[2] 郭亮[2] 梁华山[2] 田凤 邬堂春[3] 

机构地区：[1]山西医科大学公共卫生学院毒理学教研室,太原030001 [2]太钢(集团)有限公司疾病预防控制中心 [3]华中科技大学同济医学院职业医学研究所

出　　处：《中华劳动卫生职业病杂志》2007年第7期394-397,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家重点基础研究规划项目(2002CB512905);国家自然科学基金项目(30371204);山西省自然科学基金项目(20041103)

摘　　要：目的研究焦炉逸散物对焦炉工人外周血淋巴细胞热休克蛋白72(Hsp72)水平的影响及与DNA损伤的关系,探讨Hsp72在细胞损伤保护中的意义。方法选取267名焦炉工人和30名对照,以Western blot法检测外周血淋巴细胞Hsp72的水平,彗星试验检测DNA损伤水平,个体采样采集作业环境空气样本,高效液相色谱法测定环境苯并[a]芘浓度。结果高暴露组工人外周血淋巴细胞Hsp72水平(G±S_G)和Olive尾矩(G±S_G)分别为1.24±0.42和4.49±1.24,明显高于低暴露组(1.01±0.35和2.99±1.10)和对照组(0.85±0.34和2.40±1.00),差异有统计学意义(P<0.05)。以全体研究对象Hsp72中位数为界值,将研究对象分为Hsp72高表达组和低表达组,Hsp72高表达的人数相对比率在对照组、低暴露组、高暴露组分别为36.7%、43.1%和58.3%,呈逐渐增高趋势,差异有统计学意义(P=0.003)。在Hsp72高表达组,Hsp72表达水平随着暴露等级升高而升高,其中高暴露组与对照组的差异有统计学意义(P<0.05),但各组之间Olive尾矩的差异无统计学意义(P>0.05)。在Hsp72低表达组中,各组Hsp72水平的差异无统计学意义(P>0.05),而Olive尾矩随暴露等级升高而升高,其中高暴露组与低暴露组和对照组比较,差异有统计学意义(P<0.01),且明显高于Hsp72高表达组的高暴露组工人。高暴露组Hsp72水平与Olive尾矩呈负相关(r=-0.503,P<0.01)。结论焦炉逸散物可诱导焦炉工人外周血淋巴细胞Hsp72水平升高,Hsp72对细胞DNA损伤具有保护作用。Objective To investigate the relationship between heat shock protein 72 （Hsp72） and DNA genetic damage in peripheral blood lymphocytes of coke oven workers and the role of Hsp72 in protection of cells from genetic damage induced by coke oven emissions. Methods Two hundred and sixty-seven coke oven workers and thirty controls without occupational PAHs exposure were investigated. Benzo[a]pyrene concentrations in the ambient air individually collected were assayed by high performance liquid chromatography （HPLC）. Western Blot was used to measure Hsp72 levels and Comet assay was used to evaluate DNA damage degree. Personal information was collected by questionnaire. Results The Hsp72 level （G±SG） and olive comet tail moment （G±SG） of peripheral blood lymphocytes in high-exposure workers （1.24±0.42 and 4.49±1.24） were significantly higher than those in low-exposure workers （1.01±0.35 and 2.99±1.10, P〈0.05） and control （0.85±0.34 and 2.40±1.00, P〈0.05） respectively. The Hsp72 median level of all subjects was used as the limit to divide subjects into high Hsp72 level group and low Hsp72 level group. The rate with high Hsp72 level was 36.7%, 43.1% and 58.3% in control, low exposure and high exposure workers respectively and had a rising tendency following exposure level （P=0.003）. In high Hsp72 level group, Hsp72 level in high exposure workers was significantly higher than that in control （P〈0.05）, and there was a rising tendency along with the increase of exposed levels. But the olive comet tail moment had no significant difference among three exposed groups （P〉0.05）. In low Hsp72 level group, there no difference among three exposed groups about Hsp72 levels. The olive comet tail moment in high exposure workers was significantly higher than that in low exposure workers and control （P〈0.01） and high exposure workers in Hsp72 positive group, and there was a rising tendency along with the increase of exposed levels. Hsp72 levels had strong negative correlation w

关 键 词：焦炉工人 焦炉逸散物 热休克蛋白72 DNA损伤 彗星试验 

分 类 号：R131[医药卫生—劳动卫生]