4个绵羊品种MHC-DRB3基因外显子2的多态性分析  

Polymorphisms of Exon 2 of MHC-DRB3 Gene in Four Sheep Breeds

作　　者：魏丽君[1] 石国庆[2] 王晓申[3] 柳楠[1] 管峰[4] 代荣[2] 任航行[2] 

机构地区：[1]山东莱阳农学院,青岛266109 [2]新疆农垦科学院畜牧所,石河子832000 [3]云南农业大学,昆明650000 [4]中国计量学院生命科学学院,杭州310018

出　　处：《中国畜牧兽医》2007年第7期63-66,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山东省博士基金(V610543)

摘　　要：采用PCR-RFLP方法分析4个绵羊品种（阿勒泰羊、中国美利奴肉用多胎品系、湖羊和陶塞特羊）MHC-DRB3基因第2外显子的多态性,并进行聚类分析以及基因杂合度和多态信息含量的计算。结果表明：在绵羊MHC-DRB3基因第2外显子第122、154、168、220和241 bp碱基处表现出多态性;χ^2适合性检验结果表明,4个绵羊群体MHC-DRB3基因的第2外显子的PstⅠ酶切位点达到了Hardy-Weinberg平衡状态,TaqⅠ和HaeⅢ酶切位点多态性均未达到Hardy-Weinberg平衡状态;4个绵羊群体基因杂合度和多态信息含量值分别在0.693-0.774和0.662-0.738之间,表明4个绵羊品种具有丰富的遗传多样性;聚类结果表明,中国美利奴肉用多胎羊和湖羊亲缘关系最近,阿勒泰羊和陶塞特羊关系较远,与实际选育过程一致,MHC-DRB3多态性可作为绵羊育种标记进一步研究。In this study, the exon 2 of MHC-DRB3 gene was amplified by PCR and a uniform fragment of 285 bp was obtained in four sheep breeds（Aletai sheep, China merino sheep and hu sheep, Dorset sheep）. The 285 bp PCR product was digested with restriction endomuclease Pst Ⅰ ,Taq Ⅰ and HaeⅢ, and genetic polymorphism were investigated by PCR-RFLP. The results indicated that it had polymorphisms in the 122^th, 154^th, 168^th, 220^th and the 23^Th. The χ^2 tests showed that Pst Ⅰ restriction enzyme sites were Hardy-Weinberg equilibrium in all sheep population, Taq Ⅰ and HaeⅢ restriction enzyme sites were not Hardy-Weinberg equilibrium in four sheep population. The Gene heterozygosity is 0. 693-0. 774 and polymorphism information content is 0. 662-0. 738 in four sheep breeds, the results showed that the genetic diversity of four sheep breeds are rich; Clustering analysis showed that China merino sheep had the closest genetic relationship with hu sheep, further with Aletai sheep and Dorset sheep,it is the same with breed selection,and can do further reacher on the Polymorphisms of MHC-DRB3 Gene as the sheep breeding marker.

关 键 词：绵羊 MHC-DRB3 PCR-RFLP 遗传多态性 

分 类 号：Q754[生物学—分子生物学]