兔高脂血症与血管内皮功能的关系  

Relationship of hypercholesterolemia with vascular endothelium function in rabbits

作　　者：秦玲[1] 陈冬梅[1] 黄可欣[2] 刘少岩[3] 王晓美[1] 

机构地区：[1]吉林大学第一临床学院心内科,吉林长春130021 [2]吉林大学基础医学院组织学与胚胎学教研室,吉林长春130021 [3]长春市中心医院心内科,吉林长春130051

出　　处：《基础医学与临床》2007年第7期767-771,共5页Basic and Clinical Medicine

基　　金：吉林省科技厅科技发展计划项目(20050408-3)

摘　　要：目的观察一氧化氮(NO)和细胞间黏附分子-1(ICAM-1)在高脂血症及动脉粥样硬化(AS)动物模型中的表达,探讨高脂血症及AS与血管内皮功能和ICAM-1的关系。方法采用高脂饮食饲养家兔建立高脂血症和AS动物模型,正常饮食组为对照组;两组动物于0、8和16周分别取静脉血,检测血清中胆固醇(TC)、低密度脂蛋白(LDL)、NO和ICAM-1水平,并观察TC和LDL与NO和ICAM-1的相关性;16周后处死动物,免疫组化法和RT-PCR检测ICAM-1在主动脉粥样硬化斑块中的表达。结果饲养8周时,高脂饮食组血清TC和LDL明显增加(P<0.01),NO和ICAM-1无明显变化;饲养16周时,NO水平明显下降(P<0.01),ICAM-1水平明显增高(P<0.01)。TC和LDL与NO呈负相关,与ICAM-1呈正相关。结论高脂血症及AS造成血管内皮功能失调,导致NO和ICAM-1的异常表达;NO和ICAM-1参与了AS的形成。Objective To study the expression of nitricoxide （NO） and intercellular adhesion molecule-1 （ICAM-1） in the animals with hypercholesterolemia and atherosclerosis （AS） in order to investigate the relationship of hyperchol- esterolemia with AS and vascular endothelium function and ICAM-1. Methods Fed rabbits with high-cholesterol diet to establish the models of hypercholesterolemia and AS, and the control group was fed with normal diet; Blood was collected from vein in 0, 8 and 16 weeks in two groups, and the concentrations of TC, LDL, NO and ICAM-1 were measured; The correlation among TC, LDL and NO, ICAM-1 was observed： After 16 weeks, the rabbits were put to death, and immunohistochemistry analysis and RT-PCR were performed to measure the expression of ICAM-1 in the aortas. Results TC and LDL in 8 weeks were higher in the high-cholestrol diet group, but NO and ICAM-1 had no change; NO decreased and ICAM-1 increased in 16 weeks （P 〈0. 01 ） ; TC and LDL were negatively correlated with NO and positively correlated with ICAM-1 ; The expression of ICAM-1 in the high-cholesterol diet group was higher than that of control group （P 〈0. 01 ）. Conclusion Hypercholesterolemia and AS lead to vascular endothelium disorder and abnormal expression of NO and ICAM-1 ; NO and ICAM-1 may contribute to the formation of AS.

关 键 词：高脂血症 一氧化氮 细胞间黏附分子-1 动脉粥样硬化 

分 类 号：R589.2[医药卫生—内分泌]