ERK1/2和TGF-β1在自发性高血压大鼠心脏中的表达上调  

Over-expression of extracellular signal-regulated kinase 1/2 and transforming growth factor β1 in heart of spontaneous hypertensive rat

作　　者：赵琳[1] 张建中[1] 刘泉 

机构地区：[1]宁夏医学院病理学教研室,宁夏银川750004

出　　处：《基础医学与临床》2007年第7期781-784,共4页Basic and Clinical Medicine

摘　　要：目的探讨自发性高血压大鼠(SHR)心脏细胞外信号调节激酶1/2(ERK1/2)和转化生长因子β1(TGF-β1)表达的意义。方法采用免疫组化和Western blot方法检测心脏ERK1/2和TGF-β1的表达。结果在SHR8、SHR16、SHR20三个周龄组中,心肌间小动脉内皮细胞中的ERK1/2阳性率分别为15.38%、76.97%和72.72%,SHR16和SHR20组心肌间血管平滑肌细胞(VSMC)ERK1/2染色阳性率为5.49%和6.83%,均显著高于对照组(P<0.05)。在SHR16和SHR20组中ERK1/2蛋白含量明显高于对照组(P<0.05);心脏中TGF-β1含量明显高于同周龄的SD大鼠(P<0.05)。心脏中ERK1/2和TGF-β1表达量与平均动脉血压正相关(P<0.05)。结论在SHR心脏组织中TGF-β1表达增加并与ERK1/2相互作用,可能参与了心脏病变的形成。Objective To investigate the expression of extracellular signal-regulated kinase 1/2 （ERK1/2） and transforming growth factor β1 （TGF-β1） in heart of spontaneous hypertensive rat. Methods The expression of ERK1/2 and TGF-β1 was determined by immunohistochemistry and Western blot. Results In arteriole of the heart of 8,16, and 20 week-old SHR, the rate of positive ERK1/2 staining at the endothelium （ 15.38% ,76. 97% and 72. 72%, respectively） and at the vascular smooth muscle cell（VSMC） of 16 and 20 week-old SHR（5.49% and 6. 83%, respectively）were significantly higher than that of control group （P 〈 0.05 ）. The over-expression of ERK1/2 in the heart of 16 and 20 week-old SHR was also demonstrated by Western blot. The absorbance value of TGF-β1 in the heart of 16 and 20 week-old SHR was significantly higher than that in the age-matched SD rats （P 〈0, 05）. A positive relationship was found between the ERK1/2, TGF-β1 and the blood pressure （P 〈 0. 05）. Condusion In the spontaneous hypertensive rat, over-expression of TGF-β1 was induced in the heart. Interaction of ERK1/2 with TGFβ1 contributes to heart lesions.

关 键 词：高血压 心脏 细胞外信号调节激酶1/2 转化生长因子β1 血管平滑肌细胞 

分 类 号：R544.1[医药卫生—心血管疾病] Q753[医药卫生—内科学]