正常人角质形成细胞维A酸受体γmRNA在依曲替酸处理前后的改变  

Alteration of the Expression of Retinoic Acid Receptor Gamma mRNA in Normal Human Keratinocytes Before and After Acitretin Treatment

作　　者：罗素菊[1] 彭振辉[1] 郑焱[1] 徐浩翔[1] 曹振平[1] 张磐谏[1] 

机构地区：[1]西安交通大学第二医院皮肤科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2007年第7期388-390,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助项目(No.30371295;30400387);陕西省自然科学基金资助项目(No.2004C211)

摘　　要：目的研究正常人角质形成细胞维甲酸受体(RAR)γmRNA在依曲替酸处理前后的改变。方法用0.1μmol/L和1.0μmol/L依曲替酸处理正常人角质形成细胞后12h,用RT-PCR和实时荧光定量RT-PCR法检测维甲酸受体γmRNA表达的改变。结果正常人角质形成细胞可表达少量的维甲酸受体γmRNA;0.1μmol/L依曲替酸处理后,RARγmRNA的表达无明显增高,为正常对照组的1.8倍(P>0.05);1.0μmol/L依曲替酸处理后,RARγmRNA的表达明显增高,为正常对照组4.0倍(P<0.01)。结论低浓度依曲替酸(低于0.1μmol/L)对正常人角质形成细胞RARγmRNA的表达无明显影响,但较高浓度的伊曲替酸可明显诱导RARγmRNA的表达。Objective To investigate the alteration of retinoic acid receptor gamma （RARγ） mRNA expression in normal human keratinocytes after acitretin treatment. Methods After a 12-hour incubation with 0. 1μmol/L or 1.0 μmol/L acitretin in normal human keratinocytes, the expression of RARγmRNA was examined by reverse transcription polymerase chain reaction （ RT- PCR） and real-time quantitative RT-PCR. Results RARγmRNA was expressed at low levels in normal human keratinocytes and that was enhanced by 1.8-fold（ P 〉 0.05）and 4.0-fold （P 〈 0.01 ） after treatment with 0.1 μmol/L and 1 μmol/L acitretin, respectively. Conclusion Subpharmacological concentration of acitretin can not obviously upregulate RARγmRNA expression,while higher concentration induce it significantly.

关 键 词：角质形成细胞 维甲酸受体 依曲替酸 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]