不同生殖激素状态下兔卵巢、子宫钙调素免疫组化定位的研究  

Immunohistochemical Localization of Calmodulin in Ovary and Uterus of Rabbit in a Different Physiological State

作　　者：冷颖[1] 杨纫姝[1] 杨丽珍 谢衷明 

机构地区：[1]南京师范大学生物系,南京210097 [2]上海计划生育科学研究所国家计划生育药具重点实验室,上海200032

出　　处：《生殖与避孕》1997年第3期136-140,共5页Reproduction and Contraception

基　　金：国家计划生育药具重点实验室资助

摘　　要：本文采用钙调素（CaM）免疫组化定位技术，经DAB显色观察问情期兔、假孕兔、假孕后经雌、孕激素或来非司酮处理兔卵巢和子宫中CaM的分布。结果显示：不同生殖激素状态下兔卵巢（基质和黄体）、子宫（内膜及肌层）组织均可见CaM免疫反应阳性产物（显色剂DAB的棕色颗粒）存在，但其数量随激素状态而变化。卵巢中，间情期最低，假孕兔及假孕后雌、孕激素处理兔次之，假孕后米非司酮处理兔最高。子宫中，间情期最低，假孕兔次之，假孕后雌、孕激素或米非司酮处理克最高。提示CaM可能参与哺乳动物卵巢激素分泌功能的调节，并与卵巢激素对子宫生长、内膜发育等调控作用密切相关。By means of imrnunohistochemical technique and stained by DAB,the localization of calmod-ulin (CaM) in the rabbit ovary and uterus during diestrual,pseudopregnant and pseudopregnantinjected with estrogen,progesterone or Mifepristone were observed. The reaction products werebrown particles which distributed in the ovary(stroma and corpus luteum) and uterus (endometri-um and myometrium)of rabbit under different hormonal states. In the ovary,the amount of brownparticles of pseudopregnant rabbit and the pseudopregnant rabbit injected with estrogen or pro-gesterone was higher than that of diestrual rabbit,but lower than that of pseudopregnant rabbitwhich was injected with Mifepristone. In the uterus,the amount of brown particles 0f pseudopreg-nant rabbit was higher than that of diestrual rabbit,but lower than that of pseudopregnant rabbitinljected with estrogen,progesterone or Mifepristone. The results indicate that CaM may be relat-ed to the steroidogenesis of ovary and involved in the regulation of uterus,especially endomeriumby estrogen and progesterone.

关 键 词：钙调素 免疫组化定位 卵巢 子宫 激素 

分 类 号：R339.22[医药卫生—人体生理学] R392.9[医药卫生—基础医学]