醋酸棉酚与米索前列醇对离体培养的大鼠黄体细胞和人蜕膜细胞的影响  

The Effect of Gossypol and Misoprostol on Rat Luteal Cells and Human Decidual Cells in Vitro

作　　者：陈咏[1] 曹霖[1] 顾芝萍 

机构地区：[1]中科院上海药物研究所,上海200031

出　　处：《生殖与避孕》1997年第3期141-145,共5页Reproduction and Contraception

摘　　要：醋酸棉酚和米索前列醇对体外培养大鼠黄体细胞和人蜕膜细胞都有明显的损伤作用，呈现量-效相关性。棉酚单用对黄体、蜕膜细胞的LD50分别为1．27±0．09μg／ml和3．06±0．23μg／mg；但与来索5μg／ml（在黄体细胞），10μg／ml（在人蜕膜细胞）合用时，LD50分别降至0．89±0．25μg／ml和1．88±026μg／ml；与单用棉酚相比，有显著性差异。单用米索对黄体、蜕膜细胞的LD50各为14．29±1．29μg／ml和24．37±4．49μg／ml；但与棉酚0．5μg／ml（在黄体细胞），1．0μg／ml（在蜕膜细胞）合用时，分别下降为8．79±2．18μg／ml和17.29±1．56μg／ml，与单用米索相比，均有统计学差异，显示两药合用的协同效应。Gossypol and misopr0stol could directly damaged the luteal and decidual cells cultured in vitro. The LD50 of gossrool alone to luteal and decidual cells were 1. 27±0. 9 μg/ml and 3. 06±0. 23μg/ml, repectively;however when combined with misoprostol (to luteal cells 5 μg/ml,to decidualcells 10 μg/ml),the LD50 of gossypol significantly decreased to 0.89±0. 25μg/ml and 1.88±0. 26μg/ml, respectively. The LD50 of misoprostol alone to luteal and decidual cells were 14. 29±1. 29μg/ml and 24. 37±4.49 μg/ml respectively;but it decreased to 8. 79±2. 18 μg/ml and 17. 29± 1.86 μg/ml respectively when combined with gossypol (to luteal cells 0. 5 μg/ml,to decidual cells 1.0 μg/ml), also showing statistical difference. The results suggested that the combination ofgossypol with misoprostol had synergistic cytotoxic effect on the luteal and decildual cells in vitro.

关 键 词：醋酸棉酚 米索前列醇 黄体细胞 蜕膜细胞 

分 类 号：R979.22[医药卫生—药品]