Lck在狼疮肾炎肾小管上皮细胞中表达的研究  

Expression of Lck in renal tubule epithelial cells of lupus nephritis

作　　者：邓英辉[1] 李清刚[2] 任崇余[3] 徐秀红[2] 

机构地区：[1]首都医科大学宣武医院肾内科,北京100053 [2]首都医科大学附属北京友谊医院肾内科 [3]中国医学科学院中国协和医科大学基础医学院生理室

出　　处：《中华风湿病学杂志》2007年第7期402-405,F0003,共5页Chinese Journal of Rheumatology

摘　　要：目的观察狼疮肾炎病理过程中淋巴细胞特异性蛋白酪氨酸激酶(Lck)在肾小管上皮细胞内的表达及白细胞介素-2(IL-2)对其表达的影响。方法体外培养6周龄BXSB狼疮小鼠的近端肾小管上皮细胞,给予IL-2刺激,采用反转录聚合酶链反应(RT-PCR)方法及免疫印迹法检测肾小管上皮细胞中Lck mRNA和蛋白的表达,观察IL-2对Lck表达水平的影响。同时用免疫组织化学方法观察Lck蛋白在狼疮肾炎小鼠和人类肾脏组织标本肾小管上皮细胞内的表达情况。结果体外培养的6周龄BXSB狼疮小鼠近端肾小管上皮细胞中仅有微量LckmRNA及蛋白的表达,IL-2刺激后表达水平显著增加(P<0.05)。免疫组织化学方法显示:正常BALB/C小鼠、未发病的狼疮小鼠和人类微小病变性肾病肾脏组织的肾小管上皮细胞内均未发现明显的Lck蛋白表达,而已发病的16周龄狼疮肾炎小鼠和人类Ⅳ型狼疮肾炎肾脏组织标本的肾小管上皮细胞内则有明显的Lck蛋白表达。结论IL-2可活化肾小管上皮细胞并诱导Lck表达,Lck可能作为细胞因子或炎症因子的重要信号分子,在小管-间质性炎症及狼疮肾炎病理过程中发挥着重要作用。Objective To investigate the Lymphocyte-specific protein-tyrosine kinase （Lck） gene expression in the renal tubule epithelial cells （TECs） of lupus nephritis, and the effect of interlenkin-2 （IL-2） stimulation on its expression. Methods Proximal TECs derived from 6 weeks old spontaneous systemic lupus erythematosus （SLE） BXSB mice were exposed to IL-2 （100 U/ml）, the expression of Lck mRNA and protein was examined by reverse transcription-polymerase chain reaction （RT-PCR） and immunoblotting respectively. The difference of Lck gene expression before and after IL-2 stimulation was investigated. The expression of Lck protein in TECs of renal tissues of BXSB mice and human with lupus nephritis was observed through immunohistochemistry. Results The expression of Lck mRNA and protein was very low in cultured TECs of 6 weeks old BXSB mice, but increased sharply after IL-2 stimulation （P〈0.05）. Immunohistochemistry showed that there was no Lck protein found in the TECs of kidney tissue of normal 6 weeks old BALB/C mice and BXSB mice without lupus nephritis and human with minimal change nehropathy, but evidently increased in the TECs of renal tissue of 16 weeks old BXSB mice with lupus nephritis and human with type Ⅳ lupus nephritis. Conclusion IL-2 could induce Lck expression obviously in TECs, Lck may be an important signal molecule in tubulointerstitial inflammation and lupus nephritis pathogenesis.

关 键 词：狼疮肾炎 肾小管 淋巴细胞特异性蛋白质酪氨酸激酶p56(Lck) 白细胞介素2 小鼠 

分 类 号：R593.242[医药卫生—内科学]