脊髓腹外侧索强直刺激在运动神经元诱发的长时程增强  

Long-term potentiation of synaptic transmission in motoneurons induced by tetanic stimulation of ventrolateral funiculus of neonatal rat spinal cord in vitro

作　　者：苏彦艳[1] 江潇[1] 汪萌芽[1] 

机构地区：[1]皖南医学院细胞电生理研究室,安徽芜湖241001

出　　处：《皖南医学院学报》2007年第3期161-164,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(050430401);安徽省自然科学基金项目(30270366)

摘　　要：目的:探索对脊髓腹外侧索(VLF)进行强直刺激,在运动神经元(MN)能否诱发突触传递的长时程可塑性变化。方法:应用新生大鼠脊髓切片MN细胞内记录技术,观察VLF强直刺激对在MN诱发的兴奋性突触后电位(VLF-EPSP)的影响。结果:在稳定记录到VLF-EPSP的8个MNs,给予同侧VLF强直电刺激,其中有2个MNs的VLF-EPSPs幅度增大到基础值的120%以上,且维持时间超过1 h,可以定义为长时程增强(LTP)。结论:通过强直刺激VLF,可在新生大鼠脊髓MN诱发出突触传递的长时程增强,显示了长时程突触可塑性变化。Objective： To investigate whether long-term plasticity of synaptie transmission could be induced in rnotoneuron （MN） through tetanie stimulation of ventrolateral funiculus （VLF） of spinal cord slices. Methods： The intracellular recordings were performed from MNs in neonatal rat （8-14 days old） spinal cord slices, and the electrophysiological parameters of excitatory postsynaptic potential （VLF-EPSP） in MN induced by VLF stimulation were recorded before and after tetanic stimulation of VLF. Results：After tetanic stimulation of VLF in 8 MNs,the amplitude of the VLF-EPSPs in 2 MNs increased to a rnaximum of 120% of the control level and both of the increase lasted for more than 1 hour, which could be referred to as long-term potentiation （LTP）. Conclusion：Long-term potentiation of synaptic transmission in MN of neonatal rat spinal cord can be induced by tetanic stimulation of VLF in vitro.

关 键 词：运动神经元 长时程增强 腹外侧索 学习记忆 

分 类 号：R338.1[医药卫生—人体生理学]