毛细管电泳法手性拆分苯磺酸氨氯地平及其光学纯度分析  

Chiral separation and optical purity analysis of amlodipine by capillary electrophoresis

作　　者：刘英华[1] 赵星洁[1] 杨晓华[1] 贾玉英 

机构地区：[1]河北科技大学河北省分析测试研究中心,河北石家庄050018 [2]河北维尔康制药有限公司质检部,河北石家庄050031

出　　处：《河北工业科技》2007年第4期195-197,211,共4页Hebei Journal of Industrial Science and Technology

摘　　要：采用毛细管电泳法拆分苯磺酸氨氯地平手性对映体并检测其光学纯度,选择CM-β-CD为手性选择剂,考察了CM-β-CD浓度和缓冲液pH值对手性分离的影响,并对分离条件进行了优化。实验结果表明,在不添加任何有机改性剂的条件下,含10 mmol/L CM-β-CD的50 mmol/Ltris-磷酸缓冲液(pH值为2.5)可使对映体在短时间(7.22 min)内完成基线分离,分离度(RS)达4.3。A capillary electrophoresis method is adopted for chiral separation and optical purity analysis of amlodipine. CM-β-CD is selected as a chiral selector. The influences of concentration of CM-β-CD and pH value of buffer are considered and the separation conditions are optimized. The running buffer is 10 mmol/L carboxymethylated-β-cyclodextrin in 50 mmol/L trisphosphate（pH=2.5） ,which makes amlodipine resolved in 7. 22 min with no organic modifiers and Rs is 4.3.

关 键 词：毛细管电泳 苯磺酸氨氯地平 手性拆分 光学纯度 

分 类 号：O657.8[理学—分析化学]