松材线虫快速检测试剂盒研制  

Development of Quick Detection Kit for Bursaphelenchus xylophilus

作　　者：陈凤毛[1,2] 叶建仁[1] 吴小芹[1] 汤坚[3] 

机构地区：[1]南京林业大学森林资源与环境学院 [2]安徽省林业厅,安徽合肥230031 [3]安徽省林业厅

出　　处：《南京林业大学学报（自然科学版）》2007年第4期133-135,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：安徽省林业厅与南京林业大学合作研究项目(2002-2005);江苏省博士后基金计划项目(2005-2007)

摘　　要：采用引物对K09F2/R对枯死松树内分离的松材线虫、拟松材线虫及其他线虫进行检测,结果表明,所有松材线虫株系均扩增出一条340bp的清晰、明亮的条带。而拟松材线虫、其他线虫均无扩增产物。利用引物对K09F2/R构建了松材线虫检测试剂盒。采用该试剂盒可使整个检测过程不超过2.5h;该试剂盒检测灵敏度为1/7条线虫,达到了对幼虫成功鉴定的目的,且检测结果便于判读,检测费用较低。Bursaphelenchus xylophilus, B. rnucronatus and the other nematodes separated from dead pine .were detected by using primer K09F2/R. The results indicated that the PCR product of all B. xylophilus isolates had a clear and bright fragment about 340bp. But B. mucronatus and other nematodes had not any fragments. So pairwise K09F2/R was used for constructing identification kit of B. xylophilus. It took a short time to identify nematodes by adopting this kit. The time of total detection process was within two and half hours. Besides, the sensitivity of this kit reached to 1/7 of a nematode. The detection result is easy to estimate, and the cost of detection is quite low. This kit is a rapid, reliable and convenient means for detecting B. xylophilus.

关 键 词：松材线虫 快速检测 试剂盒 

分 类 号：S763[农业科学—森林保护学]