锁阳抗缺氧活性部位的药理作用及机制研究  

Pharmacological Effects and Mechanism of Anti-hypoxia Components from Cynomorium songaricum Rupr.

作　　者：罗军德[1] 张汝学[2] 贾正平[2] 李茂星[2] 王娟[2] 胡静[2] 

机构地区：[1]兰州大学生命科学院,兰州730000 [2]兰州军区兰州总医院,兰州730050

出　　处：《中药新药与临床药理》2007年第4期275-279,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：全军"十一五"面上项目(06MB101);兰州军区医药卫生科研基金资助项目(LXH-2005012)

摘　　要：目的筛选锁阳中具有抗缺氧作用的活性部位,并探讨其可能的作用机制。方法采用部位提取和大孔树脂-乙醇梯度洗脱的方法对锁阳中抗缺氧活性部位进行分离,复制常压密闭缺氧模型、高原低压缺氧模型,测定大脑含水量、脑和心肌中SOD活性、MDA含量、总蛋白及脑中乳酸含量。结果锁阳水提物和醇提物均能延长小鼠缺氧存活时间。锁阳水提取物的大孔树脂95%乙醇洗脱物(部位Ⅲ)能显著延长小鼠缺氧存活时间,减轻缺氧状态下小鼠大脑水肿,提高小鼠脑、心肌SOD活性,降低脑和心肌中MDA的含量,减少脑中乳酸的积累,增加心肌蛋白含量。结论锁阳有提高小鼠缺氧耐受的作用,其水提物部位Ⅲ具有较强的抗缺氧活性,能预防高原缺氧对脑与心肌的损伤,这与其提高缺氧时脑和心肌的SOD活性,防止膜脂质过氧化、减轻缺氧时脑中乳酸积累和增加心肌蛋白有关。Objective To screen the anti -hypoxia components from Cynomorium songaricum Rupr. and to explore its possible anti -hypoxia mechanisms. Methods The anti -hypoxia components of Cynomorium songaricum Rupr. were extracted and separated by fraction extract and macroporous resin -alcohol gradient elution. The anti -hypoxia components were screened with the closed normobaric hypoxia mice model or under altitude low - pressured hypoxia. The content of water and lactic acid in cerebral tissue, SOD activity, malondialdehyde （MDA） and protein content in both cerebral and cardiac muscle tissue were measured. Results The survival time of mice under ordinary pressure is significantly longer in the three Cynomorium songaricum Rupr. treated groups than that of blank control group. Furthermore, Part Ⅲ （from water extract and 95 % alcohol elution through macroporous resin） from the alcohol extract showed the similar effect on increasing the survival time in mice . After administration of Part Ⅲ, the SOD activity in cerebral and cardiac muscle tissue exposed to hypoxia was increased, the MDA content decreased. Meanwhile, the content of water and lactic acid in cerebral tissue was also decreased while the content of protein in cardiac muscle tissue was increased. Conclusion Cynomorium songaricum Rupr. can enhance the capacity of hypoxia tolerance. Part Ⅲ has the strong anti - hypoxia effect and can reduce the damages of cerebral and cardiac muscle tissue induced by altitude low - pressured hypoxia. The mechanism may be related to the increase of the SOD activity and inhibition of membrane lipid peroxidation in both cerebral and cardiac muscle tissue, as well as to the decrease of the accumulation of lactic acid in brain tissue and the increase of the protein contents in cardiac muscle tissue.

关 键 词：锁阳 抗缺氧 脑含水量 超氧化物歧化酶 丙二醛 乳酸 

分 类 号：R285.5[医药卫生—中药学]