环维黄杨星D对心肌缺血大鼠心肌细胞凋亡的影响  

Effects of Cyclovirobuxinum D on Myocardium Cell Apoptosis of Myocardial Ischemia Rats

作　　者：周玖瑶[1] 孙毅东[1] 邓素坚[1] 谭永恒[1] 黄桂英[1] 

机构地区：[1]广州中医药大学药理学教研室,广州510006

出　　处：《中药新药与临床药理》2007年第4期285-288,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广州市科技计划项目(2006Z3-E5021)

摘　　要：目的观察环维黄杨星D(Cvb-D)对异丙肾上腺素(Iso)致心肌缺血模型大鼠心肌细胞凋亡的影响。方法给大鼠灌服Cvb-D0.55,1.1和2.2mg/kg,连续21d,以Iso复制心肌缺血性模型大鼠,光学显微镜下观察各组大鼠的心肌组织病理学变化,采用末端标记TUNEL法测定Cvb-D对心肌细胞凋亡的影响,以凋亡细胞数占细胞总数的平均百分比表示。结果Cvb-D可显著减轻Iso诱发的心肌缺血致心肌肌纤维排列紊乱,坏死细胞胞浆溶解,坏死间质区炎性细胞浸润等病理改变;Cvb-D可显著抑制Iso诱发的心肌缺血性模型大鼠心肌细胞凋亡。结论Cvb-D具有抗Iso致心肌缺血,改善心肌缺血致细胞凋亡作用。Objective To observe the effects of cyclovirobuxinum D（Cvb - D ） on the myocardium cell apoptosis of myocardial ischemia rats. Methods The rats were given Cvb - D 0.55, 1.1 and 2.2 mg/kg orally per day for 21 days respectively. The myocardial ischemia model was induced by isoprenaline intraperitoneal injection. Myocardium tissue was observed under light microscope and end labelling TUNEL method was used to determine the cell apoptosis. Then the average percentage of apoptosis cell was figured out to evaluate the effect of Cyb - D. Results Cvb - D obviously lightened isoprenaline - induced myocardial pathological changes such as turbulence of myocardial fiber, plasmolysis of necrotic cell, inflammatory cell infiltration of necrotic interstitial region. Cvb - D remarkably inhibited myocardium cell apoptosis of myocardial ischemia rats caused by isoprenaline. Conclusion cell apoptosis caused by myocardial ischemia.

关 键 词：环维黄杨星D 异丙肾上腺素 心肌缺血 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]