三七总皂甙对血管平滑肌细胞凋亡及C-myc基因表达的影响  

Effects of PNS on the Apoptosis and Expression of C-myc of Vascular Smooth muscle Cells

作　　者：张延斌[1] 周永兰[1] 杨煜[1] 潘德锋[1] 李东野[1] 

机构地区：[1]徐州医学院附属医院心内科,221000

出　　处：《医学研究杂志》2007年第8期120-121,共2页Journal of Medical Research

摘　　要：目的观察PNS对体外培养的VSMC凋亡及C-myc基因表达的影响。方法体外培养兔胸主动脉血管平滑肌细胞,在培养基中加入不同浓度的PNS,采用流式细胞仪检测和电镜观察细胞凋亡率,细胞免疫组化方法检测C-myc蛋白。结果PNS组细胞凋亡率明显增加,电镜下呈典型细胞凋亡形态学改变。该组C-myc蛋白的表达也增加。结论PNS可诱导VSMC的凋亡,促进C-myc基因的表达可能是其作用环节。Objective To observe the effects of panax notoginseng saponins on the apoptosis and expression of C - myc of vascular VSMC in vitro. Methods VSMC derived from rabbit aorta were cultured in vitro and different concentrations of PNS were added in experimental groups. VSMC apoptosis was evaluated with cell ultrastructure under electron microscopy and flow cytometry. Expression of C - myc was observing by immunocytochemical method. Results The ratio of apoptosis and the expression of C - myc in PNS groups were high than control group. Conclusions PNS can increase the ratio of VSMC apoptosis which may be related the expression promoting of C - myc.

关 键 词：三七总皂甙 血管平滑肌细胞 凋亡 C—myc 

分 类 号：R285.5[医药卫生—中药学]