DNA3′,5′-磷酸二酯键骨架结构稳定的热力学基础分析  

Analyse on the Thermodynamical Basis of 3′,5′-PhosphodiesterLinkage to Structural Stability of DNA

作　　者：徐霆[1] 赵茜怡[1] 吕庆章[1] 杨林[1] 徐存拴 

机构地区：[1]河南师范大学化学与环境科学学院,新乡453007 [2]河南师范大学生命科学学院,新乡453007

出　　处：《河南科学》2007年第4期554-556,共3页Henan Science

基　　金：国家基础研究重大项目973前期研究专项(2006CB708506)

摘　　要：为了解大自然选择3′,5′-磷酸二酯键作为DNA结构骨架的必然性,从热力学角度模拟和计算了磷酸、2′-脱氧核糖、胞嘧啶及它们在不同位置结合形成的磷酸-2′-脱氧核糖、胞嘧啶-2′-脱氧核糖、磷酸-2′-脱氧核糖-胞嘧啶的能量,发现3′-磷酸-2′-脱氧核糖、5′-磷酸-2′-脱氧核糖、1′(-4-胞嘧啶)-2′-脱氧核糖、3′-磷酸-1′(-4-胞嘧啶)-2′-脱氧核糖、5′-磷酸-1′(-4-胞嘧啶)-2′-脱氧核糖的能量比其他相应形式结合物的能量低.表明磷酸与2′-脱氧核糖形成的3′,5′-磷酸二酯键有作为DNA结构骨架的热力学依据.To understand why 3′, 5′-phosphodiester bond is ultimately chosen by the nature as the DNA structural framework,in this article,the energy of the phosphoric acid, 2′-ribodesose, cytosine, as well as their compounds phosphoric acid-2′-ribodesose, 2′-ribodesose-cytosine and phosphoric acid-2＇-ribodesose-cytosine were calculated following thermodynamic method. It was found that the energy of 3′-deoxynucleotide, 5′-deoxynucleotide, 1′- deoxycytidine monophosphate, 1′, 3′-cytidine monophosphate, 1′, 5′-eytidine monophosphate is lower than that of the other resembling and non-resembling compounds, implying that there is a thermodynamical reason that the hydroxyl groups on the 2′-deoxyribose link to the phosphate groups to form DNA backbone.

关 键 词：2′-脱氧核糖 胞嘧啶 DNA结构骨架 热力学 

分 类 号：R284.1[医药卫生—中药学]