骨灵片对骨质疏松大鼠血清OPG/RANKL、PICP和相关细胞因子的影响  

Effect of Gu Ling Pian on Serum Levels of OPG/RANKL,PICP and Other Related Cytokines in Rat Models of Osteoporosis

作　　者：曹艳艳[1] 李娟[1] 杨春莉[1] 黄少慧[1] 吴湘慧[1] 赵毅[1] 

机构地区：[1]南方医科大学附属南方医院,广州510515

出　　处：《热带医学杂志》2007年第7期629-632,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(No.30371840);广东省科技计划项目(No.2003B31703)。

摘　　要：目的观察骨灵片对骨质疏松症(OP)大鼠血清OPG、RANKL及PICP和相关细胞因子(IL-6、TNF-α和IL-4)表达的影响。方法随机将大鼠分为正常对照组、模型组、骨灵片高、中、低剂量组,采用去势法建立大鼠OP动物模型,术后1个月,骨灵片组给予骨灵片,给药后3个月,采用ELISA法检测血清OPG、RANKL及PICP和IL-6、TNF-α、IL-4。结果与模型组比较,骨灵片各组的OPG、PICP值显著升高(P<0.05,P<0.01),而RANKL、IL-6和TNF-α值均显著下降(P<0.01,P<0.01,P<0.05),骨灵片高、中剂量组的IL-4值显著升高(P<0.05)。结论骨灵片可能通过上调OPG,下调RANKL的表达,影响OPG系统,并可能通过调节IL-6、TNF-α、IL-4水平,影响破骨细胞功能,抑制骨吸收,同时可能通过增加PICP表达而使I型胶原的合成增加,进而影响骨的韧性和强度,达到防治OP的目的。Objective To study the effect of Gu Ling Pian （GLP） on serum levels of osteoprotegerin （OPG）, receptor activator of nuclear factor-κB ligand （RANKL）, carboxyterminal propeptide of type Ⅰ procollagen （PICP）, and other related cytokines （IL-6, TNF-α, IL-4） in castrated rats. Methods Rats were randomly divided into sham-operated group, model group, high dosage GLP group, middle dosage GLP group and low dosage GLP group. Osteoporosis （OP） rat model was established by castration. A month after operation, GLP groups were fed with GLP. Three months after feeding, serum levels of OPG, RANKL, PICP, IL-6, TNF-α, and IL-4 were evaluated by ELISA. Results Compared to the model group, serum levels of OPG and PICP were increased significantly （P〈0.05 and P〈0.01, respectively）, while levels of RANKL, IL-6 and TNF-α were decreased significantly in the three GLP groups （P〈0.01, P〈0.01 and P〈 0.05, respectively）. And in the GLP of high and middle dosage group, serum levels of IL-4 were increased significantly （P〈0.05）. Conclusion The prevention and therapeutic effect of GLP on OP may be via the up-regulation of OPG and down regulation of RANKL, IL-6 and TNF-α. This would affect the function of osteoclast and inhibit bone resorption. On the other hand, up-regulating of PICP probably may result in the increase of type Ⅰ procollagen, which finally affected the bone tenacity and intensity.

关 键 词：骨质疏松症 骨灵片 护骨素 Ⅰ型前胶原羧基端前肽 

分 类 号：R681[医药卫生—骨科学]